Metabonomic study of rats exposed to cigarette sidestream smoke  

作　　者：练文柳 石先哲 罗嘉 任凤莲 

机构地区：[1]School of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China [2]Technology and Research Center,China Tobacco Hunan Industrial Co.,Ltd.,Changsha 410014,China [3]CAS Key Laboratory of Separation Science for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China

出　　处：《Journal of Central South University》2016年第3期535-543,共9页中南大学学报（英文版）

基　　金：Project(20805045)supported by National Natural Science Foundation of China

摘　　要：A metabonomic approach was undertaken in order to detect urinary endogenous and exogenous metabolites and to evaluate the effects of passive exposure to cigarette sidestream smoke on rats. Urinary samples from three groups of rats were determined including control rats, rats treated with blended cigarettes(nonmenthol cigarettes) and rats treated with menthol cigarettes. The total urinary 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol(NNAL), total 1-hydroxypyrene(1-HOP) and 3-hydroxybenzo[a] pyrene(3-HOBaP) were determined for assessing exposure to cigarette sidestream smoke toxins. Urinary endogenous metabolites in the three groups of rats were also analyzed and the data were processed by chemometrics. Eleven endogenous metabolites were found and identified. Their relative levels were compared among the three groups. The results show that cigarette sidestream smoke has complex effect on rats. Blended cigarette group makes difference to menthol cigarette group in the rats' urinary metabolic changes. Menthol adding to cigarettes has positive and negative effects on rats, respectively. The urinary metabolic profiling of menthol cigarette group is closer to that of control group.A metabonomic approach was undertaken in order to detect urinary endogenous and exogenous metabolites and to evaluate the effects of passive exposure to cigarette sidestream smoke on rats. Urinary samples from three groups of rats were determined including control rats, rats treated with blended cigarettes（nonmenthol cigarettes） and rats treated with menthol cigarettes. The total urinary 4-（methylnitrosamino）-1-（3-pyridyl）-1-butanol（NNAL）, total 1-hydroxypyrene（1-HOP） and 3-hydroxybenzo[a] pyrene（3-HOBaP） were determined for assessing exposure to cigarette sidestream smoke toxins. Urinary endogenous metabolites in the three groups of rats were also analyzed and the data were processed by chemometrics. Eleven endogenous metabolites were found and identified. Their relative levels were compared among the three groups. The results show that cigarette sidestream smoke has complex effect on rats. Blended cigarette group makes difference to menthol cigarette group in the rats＇ urinary metabolic changes. Menthol adding to cigarettes has positive and negative effects on rats, respectively. The urinary metabolic profiling of menthol cigarette group is closer to that of control group.

关 键 词：METABONOMICS RATS URINE endogenous metabolites exogenous metabolites 

分 类 号：R114[医药卫生—卫生毒理学]