女性非吸烟肺腺癌患者癌组织中miR-93-3p表达与EGFR突变的关系  

Relationship between miR-93-3p expression and EGFR mutation in non-smoking female patients with lung adenocarcinoma

作　　者：王玉[1] 张寰[1] 许芳秀 俞晓[3] 林虹燕 钱碧云[1,3] 

机构地区：[1]天津医科大学肿瘤医院,国家肿瘤临床研究中心,天津市肿瘤防治重点实验室,天津300060 [2]中国人民解放军第401医院 [3]上海交通大学公共卫生学院

出　　处：《山东医药》2016年第22期8-11,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81573231);天津市自然科学基金资助项目(13JCYBJC23100)

摘　　要：目的探讨女性非吸烟肺腺癌患者癌组织中表皮生长因子受体(EGFR)突变与miR-93-3p表达的关系。方法选择女性非吸烟肺腺癌患者149例,利用实时荧光定量PCR方法检测其中112例肺腺癌组织中miR-93-3p的相对表达量,应用直接测序方法检测149例肺腺癌组织中EGFR的突变情况;分析miR-93-3p表达与临床病理特征、EGFR突变及预后的关系。结果肺腺癌组织中miR-93-3p的相对表达量与临床分期有关。149例患者中,EGFR野生型78例,突变型71例;EGFR突变型患者的miR-93-3p表达高于野生型患者(P<0.01)。miR-93-3p高表达患者的预后较miR-93-3p低表达者差,但差异无统计学意义(P>0.05)。分层分析显示,年龄≤60岁的女性患者中,miR-93-3p高表达者预后较低表达者差(P<0.05)。结论 EGFR突变患者miR-93-3p表达升高;miR-93-3p可能成为女性非吸烟肺腺癌靶向治疗的新靶点,尤其是年轻女性。Objective To investigate the relationship between the mutation of epidermal growth factor receptor（ EGFR） and the relative expression of miR-93-3p in lung adenocarcinoma tissues from non-smoking female patients. Methods Totally 149 cases of non-smoking female patients with lung adenocarcinoma were selected. The expression levels of miR-93-3p in lung adenocarcinoma tissues from 112 cases of 149 patients were detected by real-time quantitative PCR. EGFR mutations were detected in 149 patients using DNA sequencing,and then we analyzed correlations of miR-93-3p expression with the clinicopathological features,EGFR mutation and prognosis. Results The relative expression of miR-93-3p was associated with the clinical stage. The miR-93-3p EGFR mutations were found in 71 of 149 patients（ 47. 65%）. The relative expression of miR-93-3p in patients with EGFR mutations were higher than that of patients with wild type（ P 0. 01）.There was no significant difference in prognosis between the patients with high and low expression of miR-93-3p,although the patients with high expression showed a poorer prognosis（ P 0. 05）. Stratified analysis revealed that for patients younger than 60 years old,higher levels of miR-93-3 pexpression indicated worse prognosis（ P 0. 05）. Conclusion The expression of miR-93-3p is increased in patients with EGFR mutations,which suggests that miR-93-3p may be a new therapeutic target for the treatment of non-smoking female patients with lung adenocarcinoma,especially for young female ones.

关 键 词：非小细胞肺癌 女性 烟草 非吸烟 表皮生长因子受体 miR-93-3p 

分 类 号：R734.2[医药卫生—肿瘤]