铅暴露对大鼠海马组织APP和tau蛋白表达影响  

Effect of long-term lead exposure on amyloid precursor protein and tau gene expressions in rat hippocampus

作　　者：宋靖宇[1] 黎砚书[2] 陈英[2] 焦欢[2] 冯昶[2] 

机构地区：[1]华中科技大学同济医院,湖北武汉430032 [2]南昌大学公共卫生学院

出　　处：《中国公共卫生》2016年第6期778-781,共4页Chinese Journal of Public Health

基　　金：国家自然科学基金(30960324;21267017)

摘　　要：目的探讨长期铅暴露对大鼠海马组织淀粉样前体蛋白(APP)和tau基因、蛋白表达影响。方法将SPF级雌性SD大鼠随机分为3组,分别为对照组(去离子水),低、高剂量铅组(乙酸铅800、1 500 mg/L),所生雄性仔鼠分别继续饮用去离子水(对照组),300 mg/L(低剂量铅组),900 mg/L(高剂量铅组)乙酸铅溶液,分别与生后3周(PNW3)、中年期(PNW41)、老年期(PNW70)测定雄性仔鼠血、海马组织中铅含量;采用荧光定量PCR(q PCR)和蛋白印迹(WB)检测APP和tau mRNA及蛋白表达水平。结果与对照组比较,各年龄染铅组雄性仔鼠血铅、海马铅含量明显增加(P<0.05);与对照组比较,高剂量铅组PNW70雄性仔鼠海马APP mRNA和蛋白表达水平[分别为(1.398±0.827)、(1.454±0.190)]均明显上升(P<0.05);与对照组比较,低、高剂量铅组PNW3雄性仔鼠海马tau蛋白表达水平[分别为(4.850±1.257)、(5.557±1.880)]明显上升(P<0.01);对照组比较,高剂量铅组PNW70雄性仔鼠海马磷酸化tau蛋白表达水平(3.225±1.098)升高(P<0.05)。结论长期铅暴露可导致断乳期和老年期大鼠海马组织APP和tau基因与蛋白表达上调。Objective To investigate changes in amyloid precursor protein （APP） and tau gene expression in hip- pocampus of rat with long-term lead exposure. Methods Female and male （ at the ratio of 2 ： 1 ） specific-pathogen free Sprague-Dawley rats were randomly divided into three groups：control group supplied with distilled water, low lead expo- sure group （LLG） with drinking water containing lead acetate of 800 mg/L, and high lead exposure group （HLG） with drinking water containing lead acetate of 1 000 mg/L. Then the male offsprings were continuously exposed to drinking water containing lead acetate of 0 mg/L （control group） ,300 mg/L （LLG）, and 900 mg/L （HLG）mg/L and lead con- tent in blood and hippocampus tissue of the offsprings were measured at postnatal week 3 （PNW3）, postnatal week 41 （PNW41） and postnatal week 70 （PNW70） ;the mRNA and protein expressions of tau and APP in hippocampus of the offsprings were determined simultaneously with real-time quantitative PCR（qPCR） and Western blot. Results Compared with those of the control group, the lead content of blood and hippocampus were significantly increased in lead-exposed male offsprings （P 〈0. 05）. The expression of APP mRNA（ 1. 398 ±0. 827） and protein （ 1. 454 ±0. 190） were signifi- cantly up-regulated in PNW70 male hippocampus of offsprings in HLG （ P 〈 0. 05 ）. Compared with those of the control group, the expression of tau protein of the offsprings in LLG （4. 850 ± 1. 257） and HLG （5.557± 1. 880） were signifi- cantly increased at PNW3 （ P 〈 0. 01 ）, and the expression of phosphorylated tau protein （ 3. 225 ± 1. 098 ） increased in male offspring＇s hippocampus of HLG at PNW70 （ P 〈 0. 05）. Condusion Long-term lead exposure induces up-regulations of mRNA and protein of APP and tau in hippocampus of rats at PNW3 and PNW70.

关 键 词：铅暴露 大鼠 海马 淀粉样前体蛋白(APP) TAU 

分 类 号：R135[医药卫生—劳动卫生]