雌激素对去势大鼠肝脂肪变性影响及炎性机制  

Effect of estrogen on hepatic steatosis and its inflammatory mechanism in ovariectomized rats

作　　者：肖洪涛[1] 郑建彬[1] 翟木绪[1] 彭依群[2] 

机构地区：[1]淄博市妇幼保健院,山东255000 [2]中南大学代谢内分泌研究所

出　　处：《中国公共卫生》2016年第6期788-791,共4页Chinese Journal of Public Health

摘　　要：目的观察雌激素对去势大鼠肝脂肪变作用,探讨雌激素肝保护作用及可能机制。方法 6周SD雌性大鼠随机分为3组:假手术组、手术组和雌二醇组,术后1周,雌二醇组大鼠每天灌胃戊酸雌二醇(1 mg/kg)水溶液,其他组大鼠给予蒸馏水,连续12周。进行胰岛素耐量试验,采用酶联免疫法测定肝组织中肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)和单核细胞趋化蛋白-1(MCP-1)含量。苏木素-伊红染色观察肝脂肪变性和炎症状况。结果与假手术组比较,手术组大鼠体重、肝重、内脏脂肪重量[分别为(439.9±53.1)、(12.6±1.9)、(29.6±12.1)g]及血清谷丙转氨酶(ALT)、谷草转氨酶(AST)水平[分别为(57.8±5.4)、(128.4±10.1)U/L]明显升高(P<0.05);与手术组比较,雌二醇组大鼠体重、肝重、内脏脂肪重量[分别为(387.3±40.7)、(10.3±2.1)、(24.1±6.5)g]及血清ALT、AST水平[分别为(44.5±4.8)、(98.2±9.6)U/L]明显降低(P<0.05)。手术组大鼠肝小叶结构模糊,肝索排列紊乱,有广泛的脂滴形成,小叶内及汇管区可见炎症细胞浸润;雌二醇组大鼠肝小叶结构仍欠清晰,脂滴明显减少,且脂滴明显变小;与手术组比较,雌二醇组大鼠肝脂肪变程度及小叶内炎症程度显著减轻。与假手术组比较,手术组大鼠肝组织匀浆中TNF-α、IL-6、MCP-1含量[分别为(186.3±17.2)、(187.6±26.5)、(112.3±22.4)pg/m L]明显升高(P<0.05);与手术组比较,雌二醇组大鼠肝组织匀浆中TNF-α、IL-6、MCP-1含量[分别为(132.6±8.6)、(110.8±21.2)、(89.1±10.8)pg/m L]明显降低(P<0.05)。结论雌激素可减轻去势大鼠肝脂肪变性程度,其机制可能与降低肝组织炎性因子表达有关。Objective To study the effect of estrogen on hepatic steatosis and to investigate hepatic protective effect of estrogen and its possible mechanism. Methods Six weeks old female Sprague-Dawley （SD） rats were randomly di- vided into a sham operation, an ovariectomization （ OVX）, and an ovariectomization plus estradiol （ E2 ） group. One week after the OVX operation, the rats in E2 group were treated intragastrically with estradiol （ 1 mg/kg）, while those in other groups were given distilled water for 12 weeks. At the end of the experiment,insulin tolerance test was conducted. The hepatic levels of tumor necrosis factor-alpha （TNF-a）, interleukin-6 （IL-6）, and monocyte chemotactic protein 1 （ MCP- 1 ） were determined with enzyme-linked imrnunosorbent assay and hepatic steatosis and inflammation were observed with hematoxylin and eosin staining. Results The end weight, liver weight, visceral fat weight, and serum levels of alanine aminotransferase and aspartate aminotransferase were significantly higher in OVX group than in the sham operation group,but significantly lower in E2 group than in OVX group （ all P 〈 0. 05 ）. For the rats of OVX, the structure of hepatic lobule was not clear;the cords of liver cells were disordered with a wide range of lipid droplets;and lobular and periportal inflammatory cell infiltration were observed. Compared to those of the OVX group, the degree of liver steatosis and inflammation were significantly decreased in E2 group （ both P 〈 0. 01 ）. The hepatic contents of TNF-a, IL-6 and MCP-1 in OVX group were significantly higher than those in the sham operation group （ all P 〈 0. 05）. Conclusion Estrogen replacement therapy can improve the degree of liver steatosis in ovariectomized rats and reduce the expression of hepatic inflammatory factors.

关 键 词：雌激素 去势大鼠 脂肪变性 促炎因子 

分 类 号：R575[医药卫生—消化系统]