青海固沙草根际微生态体系中微生物多样性分析  

Analysis of microbial diversity of root microecosystem of Orinus kokonorica

作　　者：李红晓[1,2] 张殿朋[2] 郝雅荞 赵洪新[1,3,4] 

机构地区：[1]沈阳师范大学生命科学学院,沈阳110034 [2]北京市农林科学院植物保护环境保护研究所,北京100097 [3]浙江理工大学生命科学学院,杭州310018 [4]浙江省植物次生代谢重点实验室,杭州310018

出　　处：《沈阳师范大学学报（自然科学版）》2016年第2期227-233,共7页Journal of Shenyang Normal University:Natural Science Edition

基　　金：辽宁省科技厅科学事业公益研究基金资助项目(2014003020)

摘　　要：青海固沙草(Orinus kokonorica)是生长于干旱、高海拔极端环境,特产于青海的一种优势种禾本科植物。从青海固沙草根际采集土壤,对样品中的微生物进行了分离鉴定及微生物多样性分析。共分离得到纯培养菌株232株,隶属于15个属、23个种。数量和种类较多的是细菌,共112株,分别隶属于9个属的12个种,占可培养微生物的70%,单胞菌属占绝大部分;其次为放线菌43株,6个种,占总微生物量的27%;另含有1株酵母菌和4株真菌。该根际微生态体系中,可培养微生物Simpson多样性指数D=0.703,Shannon-Wiener指数H=13.877,Shannon-Wiener均匀度指数E=1.766,多样性等级为Ⅰ级以上。该研究结果表明:青海固沙草极端生境微生态中的可培养微生物,不仅丰富多样,而且具有对极端环境的应变机制为寻找和开发极端生境微生态的功能微生物资源提供了参考。The diversity and abundance of culturable microbiome in the root microecosystem of Orinus kokonorica,a typical and representative plant in Qinghai area,was investigated in this study.Total 232 cultured microorganisms belonged to 15 genera and 23 species were isolated and identified.112 bacteria,which was 70% cultured microorganisms,belong to 9genera and 12 species.43actinomycetes which was 27% cultured microorganisms,belong to 6species,and 1yeast strain and 4fungi strains were found.The microbial Simpson diversity index,D=0.703,Shannon-Wiener index,H=13.877,Shannon-Wiener Evenness index,E=1.766,and the level of species diversity wasⅠ.These results are shown that culturable microorganisms isolated from the root microecosystem of Orinus kokonoricais a very rich diversity of microbial taxa and has the mechanism for adapting extreme environment.

关 键 词：青海固沙草(Orinus kokonorica) 可培养微生物 微生物多样性 极端环境 

分 类 号：Q938.1[生物学—微生物学]