BDNF/TrkB在舌鳞状细胞癌中的表达及BDNF对其增殖能力的影响  

Expression of BDNF/TrkB and effect of BDNF on the proliferation ability of tongue squamous cell carcinoma

作　　者：魏秀娟[1] 吴修胤[2] 佟冬冬[1] 李静[1] 杨晓露[1] 张风河[1] 

机构地区：[1]山东大学口腔医院口腔颌面外科,山东济南250012 [2]山东省莱芜市人民医院口腔科,山东莱芜271100

出　　处：《山东大学学报（医学版）》2016年第6期50-54,共5页Journal of Shandong University:Health Sciences

基　　金：山东省科技计划(2014GGH218038)

摘　　要：目的研究脑源性神经营养因子(BDNF)及酪氨酸蛋白激酶受体B(TrkB)在舌鳞状细胞癌(TSCC)中的表达情况,并观察外源性BDNF对舌鳞状细胞癌增殖活性的影响。方法采用免疫组织化学技术检测BDNF、TrkB在50例TSCC和13例正常舌黏膜中的表达;以舌鳞癌细胞系Tca8113为研究对象,采用免疫荧光技术检测BDNF、TrkB的表达,CCK8细胞增殖实验观察外源性BDNF对舌鳞癌细胞系Tca8113增殖活性的影响。结果BDNF/TrkB在舌鳞状细胞癌组织标本中阳性表达率显著高于正常舌黏膜组织(P<0.05),且两者的表达与浸润深度及淋巴转移有关。高浓度BDNF(200 ng/m L)可显著促进Tca8113的增殖活性(P<0.05)。结论 BDNF/TrkB可能与舌鳞状细胞癌的发生发展密切相关,BDNF可显著提高舌鳞状细胞癌的增殖能力。Objective To investigate the expressions of brain-derived neurotrophic factor（ BDNF） and its primary receptor tyrosine kinase receptor B（ TrkB） in tongue squamous cell carcinoma（ TSCC）,and to explore the effect of BDNF on the proliferation of TSCC. Methods The expressions of BDNF and TrkB in 50 TSCC and 13 normal mucosa tissues were detected with immunohistochemical method. The expressions of BDNF and TrkB in human tongue squamous cell carcinoma cell lines（ Tca8113） were examined with immunofluorescence technique. Cell viability of Tca8113 was detected with CCK8 assay. Results The positive rate of staining of BDNF and TrkB in TSCC was significantly higher than that in normal tongue mucosa（ P〈0. 05）. In addition,the rate of positive expression was related to infiltration depth and lymph node metastasis. BDNF（ 200 ng / m L） significantly promoted the proliferation of Tca8113. Conclusion BDNF and TrkB may be closely associated with the genesis and development of TSCC. Moreover,BDNF may promote cell proliferation of TSCC.

关 键 词：脑源性神经营养因子 酪氨酸蛋白激酶受体B 舌鳞状细胞癌 增殖能力 

分 类 号：R782[医药卫生—口腔医学]