AEG-1抑制miR-137对非小细胞肺癌增殖调控作用  

AEG-1 Reverses the Inhibition of Non-small Cell Lung Cancer Proliferation Induced by MiR-137

作　　者：常永梅[1] 王明智[2] 孙聪[1] 颜文森[1] 刘庆峰[1] 江小运[1] 王钧[1] 

机构地区：[1]广东省第二人民医院呼吸内科,广东广州510317 [2]广东省第二人民医院心胸外科

出　　处：《中南医学科学杂志》2016年第3期279-282,共4页Medical Science Journal of Central South China

基　　金：广东省科技计划项目(2014A020212447)

摘　　要：目的探讨AEG-1在miR-137抑制非小细胞肺癌（NSCLC）增殖中的作用及机制。方法 收集2013年2月-2014年5月在本院手术治疗的65例NSCLC患者肿瘤标本,提取RNA,qRT-PCR检测NSCLC组织、A549、H460及16HBE细胞中miR-137与AEG-1的表达,统计分析NSCLC组织中miR-137与胶质细胞上调基因1（AEG-1）表达的相关性;将A549细胞根据不同转染试剂分成4组：转染miR-137 mimics及空载体组,转染miR-137mimics及AEG-1表达载体组,转染miR-137 inhibitor及siRNA无关序列组,转染miR-137 inhibitor及AEG-1 siRNA组。用Lipofectamine 2000进行转染,生长曲线实验检测转染后细胞增殖情况。结果 在NSCLC组织中miR-137与AEG-1的表达呈负相关;miR-137表达高的NSCLC细胞中AEG-1表达降低;共同转染miR-137 mimics与AEG-1过表达载体的A549细胞在48 h的细胞数高于对照组,而共同转染miR-137 inhibitor与AEG-1 siRNA的A549细胞在48 h、72 h的细胞数低于对照组。结论 miR-137与AEG-1在NSCLC组织及细胞中表达呈负相关;AEG-1逆转miR-137对NSCLC细胞增殖的作用。Objective To investigate the the function of AEG-1 （astrocyte elevated ） in the process of miR-137 inhibiting the proliferation of non-small cell lung cancer （NSCLC）. Methods Sixty five samples in our hospital from Feb. 2013 to May 2014 were collected.And RNA was extracted from these samples.QRT-PCR was conducted to detect the expres- sion of miR-137 and AEG-1 in NSCLC tissues,and A549,H460 and 16HBE cells.The correlation between miR-137 and AEG-1 in NSCLC tissues was statistically analyzed.AS49 cells were divided into four groups according to the transfection treatment：miR-137 mimics plus empty vector group, miR-137 mimics plus AEG-1 expression vector group, miR-137 inhibitor plus scramble sequence group ,and miR-137 inhibitor plus AEG-1 siRNA group.The growth curve assay was performed to detect the cells proliferation after transfection treatment. Result The expression of miR-137 is negatively corre-lated with the AEG-1 in NSCLC tissues.The expression of AEG-1 was decreased in miR-137 overexpressed NSCLC cells.The cell number of A549 co-transfected with miR-137 mimics and AEG-1 expression vector was larger than the control group in 48 h.And,the cell number of A549 co-transfected with miR-137 inhibitor and AEG-1 siRNA was smaller than the control group in 48 h and 72 h. Conclusion The expression of miR-137 is negatively correlated with the expression of AEG-1 in NSCLC tissues and ceUs;AEG-1 reversed the effect of miR-137 in the proliferation of NSCLC cell.

关 键 词：miR-137 非小细胞肺癌 胶质细胞上调基因1 增殖 

分 类 号：R734.2[医药卫生—肿瘤]