血糖波动对糖尿病大鼠氧化应激及神经病变的影响  

Effects of oscillating blood glucose level on oxidative stress and diabetic neuropathy in diabetic rats

作　　者：马香香[1] 李亚坤[1] 陈慧晓 赵志刚[1] 

机构地区：[1]郑州大学人民医院内分泌科,河南郑州450003

出　　处：《河南医学研究》2016年第6期966-968,共3页Henan Medical Research

摘　　要：目的研究血糖波动对糖尿病大鼠神经病变的影响及氧化应激的作用。方法将SD大鼠随机分为3组:正常对照组(NC组)、稳定高血糖组(DS组)、波动高血糖组(DF组)。采用链脲佐菌素腹腔注射诱发糖尿病,DF组每天定时腹腔注射胰岛素及葡萄糖,建立血糖波动模型,同时DS组给予等体积的生理盐水建立稳定高血糖模型。12周后测定坐骨神经运动传导速度(MNCV),检测血清丙二醛(MDA)、谷胱甘肽(GSH)。结果与NC组比较,DS组和DF组大鼠坐骨神经MNCV减慢(P<0.05),血清MDA增高、GSH水平下降(P<0.05);与DS组相比,DF组MNCV进一步减慢(P<0.05),MDA增高、GSH水平下降(P<0.05)。结论波动高血糖可使糖尿病大鼠神经病变加重,其机制可能与氧化应激水平增高有关。Objective To explore the effects of oscillating blood glucose on oxidative stress and diabetic neuropathy in diabet-ic rats.Methods SD rats were randomly divided into 3 groups：normal control group （NC group）,diabetes sustained hypergly-cemia group （DS group）,diabetes fluctutating hyperglycemia group （DF group）.Streptozotoein -induced diabetic model was established with SD rats and the oscillating high blood glucose animal model was induced by intraperitoneal injection of insulin and glucose at different time points every day.At the same time,DS group was given the same volume of physiological saline to establish the stable high blood glucose animal model.12 weeks later,sciatic motor nerve conduction velocity （MNCV）,blood malondialdehyde （MDA）and glutathione （GSH）were determined.Results Compared with NC group,the DS and DF groups had a significant increase of MDA and a decrease of MNCV,GSH（P 〈0.05）.There was a significant increase of MDA and a decrease of MNCV,GSH in the rats with oscillating blood glucose than those with stable high blood glucose（P 〈0.05）.Conclu-sion Oscillating high blood glucose could induce more severe neuropathy and the mechanism may be related to the increased ox-idative stress.

关 键 词：血糖波动 神经病变 氧化应激 

分 类 号：R363[医药卫生—病理学]