Synergetic effects of ciliary neurotrophic factor and olfactory ensheathing cells on optic nerve reparation(complete translation)  

作　　者：Dan-ping Yin Qing-ying Chen Lin Liu 

机构地区：[1]Department of Disease Control and Prevention, Jinan Military General Hospital [2]Medical Department, Jinan Military General Hospital [3]Department of Ophthalmology, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

出　　处：《Neural Regeneration Research》2016年第6期1006-1012,共7页中国神经再生研究（英文版）

基　　金：supported by the Dean Fund of Jinan Military General Hospital in 2015,No.2015QN02

摘　　要：At present, there is no effective treatment for the repair of the optic nerve after injury, or improvement of its microenvironment for regeneration. Intravitreally injected ciliary neurotrophic factor（CNTF） and olfactory ensheathing cells（OECs） promote the long-distance regrowth of severed optic nerve fibers after intracranial injury. Here, we examined the efficacy of these techniques alone and in combination, in a rat model of optic nerve injury. We injected condensed OEC suspension at the site of injury, or CNTF into the vitreous body, or both simultaneously. Retrograde tracing techniques showed that 4 weeks postoperatively, the number of surviving retinal ganglion cells and their axonal density in the optic nerve were greater in rats subjected to OEC injection only than in those receiving CNTF injection only. Furthermore, combined OEC ＋ CNTF injection achieved better results than either monotherapy. These findings confirm that OECs are better than CNTF at protecting injured neurons in the eye, but that combined OEC and CNTF therapy is notably more effective than either treatment alone.At present, there is no effective treatment for the repair of the optic nerve after injury, or improvement of its microenvironment for regeneration. Intravitreally injected ciliary neurotrophic factor（CNTF） and olfactory ensheathing cells（OECs） promote the long-distance regrowth of severed optic nerve fibers after intracranial injury. Here, we examined the efficacy of these techniques alone and in combination, in a rat model of optic nerve injury. We injected condensed OEC suspension at the site of injury, or CNTF into the vitreous body, or both simultaneously. Retrograde tracing techniques showed that 4 weeks postoperatively, the number of surviving retinal ganglion cells and their axonal density in the optic nerve were greater in rats subjected to OEC injection only than in those receiving CNTF injection only. Furthermore, combined OEC ＋ CNTF injection achieved better results than either monotherapy. These findings confirm that OECs are better than CNTF at protecting injured neurons in the eye, but that combined OEC and CNTF therapy is notably more effective than either treatment alone.

关 键 词：neurotrophic olfactory optic CNTF injured alone suspension regeneration intracranial injected 

分 类 号：R774.6[医药卫生—眼科]