毛竹竹青和竹黄细胞壁中阿魏酸和对香豆酸定量分析  

Quantitative Determination of Ferulic and p-Coumaric Acids in Cell Wall of Moso Bamboo Green and Bamboo Yellow

作　　者：黄曹兴[1] 何娟[1] 闵斗勇[2] 李鑫[1] 杜丽婷[3] 勇强[1] 

机构地区：[1]南京林业大学化学工程学院,江苏南京210037 [2]南京林业大学轻工科学与工程学院,江苏南京210037 [3]南京林业大学现代分析测试中心,江苏南京210037

出　　处：《林产化学与工业》2016年第3期16-22,共7页Chemistry and Industry of Forest Products

基　　金："十二五"国家科技支撑计划项目资助(2015BAD15B09);江苏省高校优势学科建设工程资助项目(无编号);国家自然科学基金资助项目(31570561)

摘　　要：采用两步中度碱水解和两步酸水解法,定量分析毛竹竹青与竹黄细胞壁中阿魏酸和对香豆酸的含量。结果表明(基于绝干物料):竹青细胞壁中含有3.08%阿魏酸和5.91%对香豆酸,其中1.62%阿魏酸和4.64%对香豆酸以酯键形式连接,1.46%阿魏酸和1.27%对香豆酸以醚键形式连接;竹黄细胞壁中阿魏酸和对香豆酸分别为3.11%和5.51%,以酯键和醚键结合的阿魏酸分别为1.71%和1.40%,以酯键和醚键结合的对香豆酸分别为4.34%和1.17%。对碱水解液中溶解的木质素进行二维异核单量子相关核磁共振(2D-HSQC NMR)表征,结果表明:竹青和竹黄碱溶木质素中以酯键存在的阿魏酸和对香豆酸可能酯化在木质素β-O-4醚键的γ位置。Moderate alkali hydrolysis and acid hydrolysis were applied to quantitatively determine the amount of ferulic and p-coumaric acid in the cell wall of Moso bamboo green and bamboo yellow.The results indicated that 3.07% of ferulic acid and5.91% of p-coumaric acid were found in the bamboo green cell wall.And 1.61% ferulic acid and 4.64% p-coumaric acid were ester-linked to the bamboo green cell wall components,while 1.46% ferulic acid and 1.27% p-coumaric acid were ether-linked to the cell wall component.For bamboo yellow,there were 3.11% of ferulic acid and 5.51% of p-coumaric acid.The amounts of ester-linked and ether-linked ferulic acid were 1.71% and 1.40%,respectively.And the amounts of ester-linked and etherlinked p-coumaric acid were 4.34% and 1.17%,respectively.Two-dimensional heteronuclear single quantum correlation nuclear magnetic resonance（2D-HSQC NMR） confirmed that the ester-linked ferulic and p-coumaric acid in the alkaline-soluble lignins of bamboo green and bamboo yellow might be esterified in the γ position of β-O-4 aryl ether bonds in the lignin.

关 键 词：竹青 竹黄 阿魏酸 对香豆酸 定量分析 2D-HSQC NMR 

分 类 号：TQ35[化学工程]