黑荆树愈伤组织培养及其原花色素的分析  

Callus Culture of Acacia mearnsii De Wilde and Analysis on Proanthocyanidin

作　　者：徐玲玲[1] 杨阳[1] 王飞[1] 

机构地区：[1]南京林业大学化学工程学院,江苏省生物质绿色燃料与化学品重点实验室,江苏南京210037

出　　处：《林产化学与工业》2016年第3期53-59,共7页Chemistry and Industry of Forest Products

基　　金：国家林业行业公益性专项(20110419);江苏省高校优势学科建设工程项目(无编号)

摘　　要：以黑荆树叶片为外植体,在MS培养基中添加不同质量浓度的细胞分裂素6-苄氨基嘌呤(6-BA)和细胞生长素2,4-二氯苯氧乙酸(2,4-D),诱导培养出愈伤组织,采用香草醛-硫酸法和Folin-Ciocalteu法测定了不同条件处理下愈伤组织中原花色素和总多酚的含量,并用电喷雾质谱(二级质谱)法对愈伤组织中原花色素组成进行定性分析。研究结果表明:添加0.25 mg/L 6-BA和2.0 mg/L 2,4-D的培养基中,黑荆树愈伤组织诱导率及生长状况较佳,且含总多酚和原花色素分别为20.19和11.10 mg/g,明显高于其他处理,适合进行增殖培养;黑荆树愈伤组织主要由单体原花色素及低聚原花色素组成,其中以单体和二聚体居多,还有微量三聚体。This paper studied proanthocyanidins in callus of Acacia mearnsii De Wilde.Callus were obtained by inoculating leaf explants on MS medium,and supplemented with the combination of 6-BA and 2,4-D.The content of total phenolic compounds and proanthocyanidins in the 12 different treatments were tested by Folin-Ciocalteu assay and vanillin assay,respectively.Proanthocyanidins in callus were characterized by ESI-MS.The results showed that the leaf explants of A.mearnsii treated with0.25 mg/L 6-BA in combination with 2.0 mg/L 2,4-D resulted in a higher rate of callus formation,better growth and maximum content of total phenolic compounds 20.19 mg/g and proanthocyanidins 11.10 mg/g,which is suitable for proliferation culture.We detected monomer,dimer and trace trimer in callus of A.mearnsii,too.

关 键 词：黑荆树 愈伤组织 原花色素 二级质谱 

分 类 号：TQ35[化学工程]