电针对肝郁型功能性消化不良大鼠代谢组学的影响  

Influence of Metabonomics of Liver Depression Functional Dyspepsia Rats on Electric Acupuncture

作　　者：张可[1] 任路[1] 李丹[1] 林卉[1] 付沈康[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847

出　　处：《辽宁中医药大学学报》2016年第6期110-114,共5页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：沈阳市科技局应用基础研究项目(F13-318-1-67)

摘　　要：目的:从情志致病角度,通过分析下丘脑γ-氨基丁酸及尿液代谢组学表达的影响,探究肝郁型功能性消化不良的发病机制。方法:选用48只SPF级Wister大鼠分为空白组、模型组、电针组和假针刺组。建立夹尾法伴辅助刺激肝郁型功能性消化不良大鼠模型。实验第15到第28天,电针组选取足三里穴、肝俞穴刺激;假针刺组在距尾尖1/3、2/3处电针刺激。观察大鼠行为学变化;计算胃内残留物率和小肠推进率检测胃肠道动力功能;采用免疫组化的方法测定下丘脑中γ-氨基丁酸的表达情况;运用高效液相色谱-质谱联用对尿液进行代谢组学研究,分析尿液样本中小分子物质波峰面积的改变情况。结果:与模型组比较,电针组γ-氨基丁酸的表达明显升高(P<0.01)且尿液代谢组中柠檬酸、琥珀酸、苯丙氨酸、α-酮戊二酸、天门冬氨酸、肉豆蔻酸的峰面积有上升的趋势(P<0.05),假针刺组无明显差异。结论:电针足三里穴、肝俞穴对治疗肝郁型功能性消化不良及胃肠神经系统有良好的调节功能,对γ-氨基丁酸有正调节作用,并可以改善尿液样本中小分子物质的代谢含量。Objective：From the perspective of emotional pathogenic,by analyzing the hypothalamus γ- aminobutyric acid and urine metabolomics affect expression,to explore the pathogenesis of functional dyspepsia liver depression. Methods：Selection of SPF 48 healthy Wistar rats were divided into control group,model group,electric-acupuncture and sham acupuncture groups. Model in the tail clamp method liver depression functional dyspepsia,based on the type of stimulation increased,random repeat daily for 14 days. Experiment fifteenth to twenty-eighth days,selection of Zusanli and Ganshu acupuncture point stimulation of electroacupuncture group,at a distance of 1/3 and 2/3 tail tip of sham acupuncture group received acupuncture.The open field test and sucrose preference of rats was measured experimentally observed behavior changes;abdominal anatomy of stomach and duodenum of rats whether organic diseases,to check the motor function of the gastrointestinal tract;methods the expression of immunohistochemical observation of hypothalamic GABA;Urine metabonomics analysis by using high performance liquid chromatography- mass spectrometry,observed the changes of small molecular substances in urine sample peak area. Results：Compared with the blank group,the model group rats significantly decreased the expression of GABA（P〈0.01）;After the treatment,the electroacupuncture group GABA expression compared with the model group increased（P〈0.01）.the rats in the model group and blank control group comparison,the urine metabolism in rats of citric acid succinic acid,phenylalanine,alpha- ketoglutaric acid,aspartic acid,myristic acid peak area was significantly different（P〈0.05）,are decreased;after the treatment,the electroacupuncture group peak area are on the rise,there is difference with the model group（P〈0.05）.Conclusion：Electric-acupuncture at Zusanli,Ganshu acupuncture point has a good regulating function for the treatment of liver functional dyspepsia and gastro intestinal nervous system,has a positive moder

关 键 词：肝郁型功能性消化不良 Γ-氨基丁酸 尿液代谢组学 

分 类 号：R573[医药卫生—消化系统]