微囊固定化葡萄糖氧化酶性能影响因素研究  

Study on the influencing factors of the performance of microcapsule immobilized glucose oxidase

作　　者：杜龙云 宋彩云 

机构地区：[1]石药集团欧意药业有限公司,河北石家庄050000 [2]烟台荣昌制药股份有限公司,河北石家庄050000

出　　处：《煤炭与化工》2016年第5期71-74,共4页Coal and Chemical Industry

摘　　要：固定化葡萄糖氧化酶因较游离葡萄糖氧化酶稳定性高、易于控制、可反复使用、回收方便、成本低廉等优点,而广泛应用在化学、生物学、生物工程、医学及生命科学等领域中。实验研究了p H值、温度、盐酸胍浓度对固定化葡萄糖氧化酶活性的影响,以及固定化葡萄糖氧化酶制备微囊时其重复使用次数及时间对固定化葡萄糖氧化酶稳定性的影响。实验结果表明,固定化葡萄糖氧化酶在p H值为4、温度为40℃时相对活性最大;随着盐酸胍浓度的增大,固定化葡萄糖氧化酶的相对活性逐渐降低,但降低幅度<游离酶;固定化葡萄糖氧化酶重复使用时,其重复使用率相对稳定;随着时间延长,固定化葡萄糖氧化酶的相对酶活性逐渐降低,但降低幅度<游离酶。Compared with immobilized glucose oxidase, free glucose oxidase has the advantages like more stable, easy to control, can be used repeatedly, easy to recycle, low cost and etc.,so it is widely used in the fields of chemistry, biology,biological engineering, medicine, life science and etc.. The effects of p H value, temperature and concentration of guanidine hydrochloride on the activity of immobilized glucose oxidase were studied, and the effects of reusing times and time on the stability of immobilized glucose oxidase were studied when microcapsule was prepared from immobilized glucose oxidase.Experimental results show that when p H value of immobilized glucose oxidase is 4 and temperature is 40 ℃, the relative activity of immobilized glucose oxidase is maximum; with the increasing of guanidine hydrochloride concentration, its relative activity is gradually decreased, but the decrease range is smaller than the free enzyme; when immobilized glucose oxidase is reused, its repetition rate is relatively stable; with time extending, the relative activity of immobilized glucose oxidase is gradually decreased, but the rate is lower than free enzyme.

关 键 词：葡萄糖氧化酶 固定化葡萄糖氧化酶 游离葡萄糖氧化酶 微囊 活性 稳定性 

分 类 号：TQ925.4[轻工技术与工程—发酵工程]