马勃多糖CGP-Ⅱ抗S_(180)肉瘤及体外抗氧化研究  

Lsolation,Purification of Acidic Polysaccharide fromCalvatia Gigantea and Its Anti-S_(180) Sarcoma Activity

作　　者：武翠玲[1] 万兵[2] 李钰娜[1] 

机构地区：[1]长治医学院生物化学教研室,046000 [2]长治医学院第二临床学院

出　　处：《长治医学院学报》2016年第3期169-171,共3页Journal of Changzhi Medical College

基　　金：山西省高等学校大学生创新创业训练项目(2015299);长治医学院科研启动基金项目(QDZ201517)

摘　　要：目的:探讨马勃酸性多糖CGP-Ⅱ抗S180肉瘤的作用及体外抗氧化作用。方法:水提醇沉法制得马勃粗多糖,再经HiTrap Capto DEAE阴离子交换层析和Sephacryl S-300 HR凝胶柱层析进一步纯化得到马勃酸性多糖CGP-Ⅱ。采用咔唑-硫酸法测得糖醛酸含量,同时建立小鼠S180肉瘤移植模型,研究CGP-Ⅱ的体内抑瘤效应。利用氧自由基吸收法(Oxygen Radical Absorption Capacity,ORAC)、DPPH法评价其体外抗氧化能力。结果:CGP-Ⅱ糖醛酸含量为17.2%,200mg/(kg·d)剂量组多糖对S180肉瘤的抑瘤率为48.4%,CGP-Ⅱ具有一定的DPPH自由基清除能力和氧自由基吸收能力。结论:酸性多糖CGP-Ⅱ具有一定的抗肿瘤活性及体外抗氧化作用,本研究为其进一步开发利用奠定了基础。Objective ：To study the anti‐S180 activity and antioxidant effects of acidic polysaccharide from Calvatia gigantea .Methods：Water extracting and ethanol precipitating methods were applied to extract polysaccharide from Calvatia gigantea .Mice tumor model was established with transplantion of sarcoma S 180 .In addition ,antioxidant activity of CGP‐Ⅱwas evaluated by DPPH free radical scavenging assay and oxygen radical absorbance capacity （ORAC） assay .Results ：The content of uronic acid in CGP‐Ⅱ was 17.2% .The inhibition rates of CGP‐Ⅱ on sarcoma S180 in mice was 48.4% ,when the dosage of CGP‐Ⅱ reached to 200 mg/（kg ？ d） .CGP‐Ⅱ showed antioxidant capacity against DPPH and oxygen radical absorption capacity .Conclusion：CGP‐Ⅱ showed obvious antitumor and antioxidant effects .This research will provide important basis for the further research and application of CGP‐Ⅱ .

关 键 词：马勃 酸性多糖 S180肉瘤 DPPH ORAC 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]