双波长叠加分光光度法测定盐酸尼卡地平  

Determination of Nicardipine Hydrochloride by Dual-wavelength Overlapping Spectrophotometry

作　　者：杨小丽[1] 李俊波[1] 白熙[1] 刘学慧[1] 杨金香[1] 

机构地区：[1]长治医学院药学系,046000

出　　处：《长治医学院学报》2016年第3期172-176,共5页Journal of Changzhi Medical College

基　　金：山西省高等学校科技创新项目(20121101);国家级大学生创新创业训练项目(2014299);长治医学院科技启动基金项目(普及项目)(QDZ201515)

摘　　要：目的:建立一种盐酸尼卡地平的双波长叠加分光光度测定方法。方法:在pH为3.4的Britton-Robinson缓冲溶液中,曙红Y(EY)与适量的盐酸尼卡地平(NP·HCl)相互作用,在最大褪色波长516nm和最大增色波长548nm处测量吸光度ΔA。结果:褪色法和增色法的摩尔吸光系数(ε)分别为1.52×105 L/(mol·cm)和3.20×104 L/(mol·cm)。采用双波长叠加法测定时,ε提高到1.84×105 L/(mol·cm),检出限为26ng/mL。结论:该法简便、快速、可靠,可用于市售盐酸尼卡地平缓释胶囊的含量测定。Objective：To develop a dual‐wavelength overlapping spectrophotometry method for the determination of Nicardipine Hydrochloride（NP？HCl） .Methods：In a Britton‐Robinson buffer solution of pH 3.4 ,Eosin Y（EY） could interact with NP？HCl . The absorbance ΔA was measured at the maximum fading wavelength （516 nm） and the maximum coloring revealing wavelength （548 nm） .Results：The molar absorptivity （ε） of fading wavelength method and revealing wavelength were 1.52 ＆#215; 105 L/（mol？cm） and 3.20 ＆#215; 104 L/（mol？cm） ,respectively .Theεincreased to 1.84 ＆#215; 105 L/（mol？cm） by using the dual‐wavelength overlapping spectrophotometry to determine the NP ？ HCl ,detection limit of proposed method was found to be 26 ng/mL .Conclusion ：The method is simple ,accurate and sensitive ,which can be applied to the determination of the content of commercial available nicardipine hydrochloride sustained release capsules .

关 键 词：盐酸尼卡地平 曙红Y 双波长叠加分光光度法 

分 类 号：R914[医药卫生—药物化学]