耐苯巴比妥钠及苯妥英钠杏仁核点燃大鼠癫痫模型的制作  

Construction of Amygdala Kindling Epilepsy Rat Model Resistant to Phenobarbital and Phenytoin Sodium

作　　者：唐太峰 伍国锋[2] 

机构地区：[1]贵阳市第二人民医院神经内科,贵州贵阳550081 [2]贵州医科大学附院神经内科,贵州贵阳550004

出　　处：《贵阳医学院学报》2016年第6期671-674,共4页Journal of Guiyang Medical College

摘　　要：目的：建立耐苯巴比妥钠（PB）及苯妥英钠（PHT）的杏仁核点燃癫痫模型大鼠的方法。方法：60只雄性健康sD大鼠制作杏仁核点燃癫痫模型，采用PHT、PB对癫痫大鼠进行耐药筛选，用放电阈值（ADT）来判断癫痫大鼠对PHT和PB的敏感性，以应用PHT或PB前的ADT值为对照（对照值ADT），用药后ADT波动范围在20％对照值ADT以内为耐药癫痫模型判断标准。结果：60只大鼠，死亡13只，47只大鼠完成杏仁核点燃实验，点燃癫痫模型大鼠24只，发作强度4—5级，癫痫点燃率51％；PB及PHT筛选出7只耐药的癫痫大鼠，耐药率29．17％；耐药大鼠用药后仍可诱发癫痫发作，用药后ADT波动在20％对照值ADT之内。结论：利用杏仁核慢点燃癫痫模型大鼠建立的耐药癫痫模型可建立耐药癫痫模型。Objective： To explore the way for construction of the amygdala kindling epilepsy rat mod- el resistant to phenobarbital （PB） and phenytoin sodium（PHT）. Methods： 60 healthy male SD rats were selected to make the amygdala kindling epilepsy model. Amygdala kindling rats were screened by PHT and PB for their drug resistance. After discharge threshold （ADT） was used to estimate their sen- sitivity to PHT and PB. The criterion of drug resistance epilepsy model was based on the variations of ADT： ADT fluctuation range under 20% of ADT control value. Results： Among the 60 rats, 13 died and 47 rats completed the amygdala kindling experiment. 24 rats were successfully established into a- mygdala kindling epilepsy rat model, attack intensity 4 ~ 5 level and epilepsy kindling rate was 51%. 7 rats with tolerance to phenobarbital sodium （PB） and phenytoin sodium（PHT） were picked, and re- sistant rate 29.17%. Drug resistant rats could still be induced to seizure of epilepsy, and ADT fluctua- tion range was under 20% of ADT control value. Conclusion： The amygdala kindling epilepsy rat model with tolerance to phenobarbital sodium （PB） and phenytoin sodium （PHT） can be successfully established.

关 键 词：癫痫 大鼠  SD 动物模型 杏仁核点燃 耐药 苯妥英钠 苯巴比妥钠 

分 类 号：R749.16[医药卫生—神经病学与精神病学]