南京地区1株猪伪狂犬病毒的分离与鉴定  

Separation and Identification of A Strain of Porcine Pseudorabies Virus in Nanjing Area

作　　者：常晨[1] 张道华[1] 唐波[1] 刘国阳[1] 华涛[1] 侯继波[1] 

机构地区：[1]江苏省农业科学院国家兽医生物制品工程技术中心,江苏南京210000

出　　处：《江西农业学报》2016年第6期82-86,共5页Acta Agriculturae Jiangxi

基　　金：江苏省农业科技自主创新基金项目[CX(11)2047];江苏省农业支撑计划(BE2012370)

摘　　要：于2013年从南京某猪场送检发病仔猪脏器中分离获得1株病毒。病样组织液上清经BHK细胞分离培养后,利用PCR和间接免疫荧光反应进行鉴定,PCR可以扩增出PRV gE基因的全长片段,且在间接免疫荧光检测中呈现阳性荧光反应。将命名为NJ株的分离病毒接种家兔,被接种的家兔很快出现瘙痒、死亡等伪狂犬病症状。与以往发表的伪狂犬病毒gE基因序列比对及进化树分析结果显示,分离株属于一个相对独立的分支。由中国兽药监察所提供的伪狂犬病阳性血清对分离株有一定的中和能力。根据实验结果推测,南京某猪场流行的伪狂犬病毒存在一定的抗原变异。A virus was isolated from affected piglet of Nanjing city in 2013. The tissue supernatant was inoculated to BHK 21 cells,a pair of primers specific for PRV gE was designed and used to amplify the corresponding DNA segments by PCR,the infected cells showed positive reaction with PRV antibodies in IFA. The rabbit infected with this isolate appeared the symptoms of pseudorabies. Sequence alignments of gE indicated that gE gene belonged to a relatively independent sub-branch in phylogenetic analysis. The positive serum of pseudorabies provided by Chinese Veterinary Drug Supervisory Institute had a certain ability to neutralize the separated strain. In conclusion,the prevalence of PRV might has antigenic variation.

关 键 词：猪 伪狂犬病病毒 分离 鉴定 GE基因 

分 类 号：S858.28[农业科学—临床兽医学]