双酶法提取低档都匀毛尖茶总黄酮工艺  

The Process Optimization of Extracting Flavonoids from Low-Grade Duyun Maojian with the Two-Enzyme Method

作　　者：刘洁[1] 李锋[1] 黄德娜[1] 吴桂玲[1] 

机构地区：[1]黔南民族师范学院化学化工学院,贵州都匀558000

出　　处：《黔南民族师范学院学报》2016年第3期114-119,共6页Journal of Qiannan Normal University for Nationalities

基　　金：贵州省科学技术联合基金项目(黔科合LH字[2014]7418);贵州省大学生创新创业训练项目(201510670010);黔南民族师范学院地方特色研究专项项目(qnsy2013T03);贵州省科学技术联合基金项目(黔科合LH字[2015]7717)

摘　　要：以夏秋季都匀毛尖为原料,利用纤维素酶和果胶酶辅助提取低档毛尖茶中总黄酮。研究酶用量、提取温度、料液比、提取时间、酶解p H 5个因素对总黄酮提取率的影响。在单因素基础上,筛选出影响总黄酮提取率显著的因素;并结合Box-behnken中心组合试验设计原理进行响应面法回归分析,最终确定酶解工艺条件为酶用量100μL、酶解p H值5.60、料液比1∶90g/m L、提取温度55℃、提取时间68min,总黄酮提取率为4.07%。结果表明,所得模型方程能较好的预测实验结果,拟合度较好。The technology of extracting flavonoids from summer and autumn low- grade Duyun Maojian is studied with the help of cellulase and pectinase. The influences of enzyme loading,extraction temperature,liquid to solid ratio,extraction time and p H are investigated on the extraction rate. On the basis of single factor test,Box- Behnken design is adopted to screen crucial factors during the extraction rate of total flavonoids as well as the optimization of experimental conditions. The optimum extraction parameters are as follows： the enzyme loading is 100 μL; the extraction time is 68 minutes; the extraction temperature is 55℃; the ratio of solid liquid is 1： 90 g / m L and the value of p H is 5. 60. The extraction rate of flavonoids is 4. 07%,which shows that statistical method is a useful tool for the experiment results with high degree of fitting.

关 键 词：绿茶 纤维素酶 果胶酶 总黄酮 响应面法 

分 类 号：Q946[生物学—植物学]