恶性肿瘤干细胞标记物CD34在鼻咽癌细胞系的表达  

CD34, a cancer stem cell marker, in nasopharyngeal carcinoma cell lines

作　　者：张军红 李青[2] 王春华 吴秀云 卢新阁 王莉菲 杨蕾蕾 王秋红 

机构地区：[1]河北省眼科医院,河北省眼科研究所病理科,河北省邢台市054000 [2]贵阳医学院附属医院骨科,贵州省贵阳市550004 [3]河北省眼科医院,河北省眼科研究所耳鼻喉科,河北省邢台市054000 [4]河北省眼科医院,河北省眼科研究所河北省眼科学重点实验室,河北省邢台市054000 [5]河北省眼科医院,河北省眼科研究所检验科,河北省邢台市054000

出　　处：《中国组织工程研究》2016年第23期3374-3379,共6页Chinese Journal of Tissue Engineering Research

基　　金：邢台市科技支撑计划(2013ZC062)~~

摘　　要：背景:在多种肿瘤中已证实CD34与肿瘤的发生、发展、复发、转移、耐药密切相关,但是CD34与鼻咽癌的关系目前还知之甚少。目的:分选鼻咽癌细胞株中CD34+与CD34-细胞的增殖和迁移能力。方法:利用流式细胞术检测CD34在鼻咽癌细胞株5-8F、6-10B、CNE1、CNE2中的表达,根据细胞表面标志物分选出CD34+与CD34-细胞,进行纯度鉴定;通过体外MTT法、平板克隆形成实验以及划痕实验分析CD34+细胞与CD34-细胞增殖和迁移能力。结果与结论:(1)4种鼻咽癌细胞株中CD34表达均恒定在0.1%-0.2%,且CD34水平与细胞的生长密度关系密切;(2)分选的CD34+细胞群中,CD34+细胞例能达到98%以上,在CD34-细胞群中未能检测到CD34+细胞;(3)CD34+细胞在第1,3,5,7天增殖速度显著高于CD34-细胞(P<0.05);(4)CD34+细胞克隆形成率显著高于CD34-细胞(P<0.05);(5)CD34+细胞向划痕区迁移的细胞明显多于CD34-细胞(P<0.05);(6)结果提示,CD34+细胞体外培养具有更高的增殖和转移能力,具有鼻咽癌肿瘤干细胞潜能。BACKGROUND： Previous research have confirmed that CD34 is closely related to oncogenesis, progress, recurrence, metastasis and drug-resistance of various cancers, but its role in nasopharyngeal carcinoma remains unclear. OBJECTIVE： To sort cells positive and negative for CD34 in nasopharyngeal carcinoma cell lines and to detect cell proliferation and migration. METHODS： Expressions of CD34 in nasopharyngeal carcinoma cell lines 5-8F, 6-10 B, CNE1 and CNE2 were detected by flow cytometry. And CD34＋ and CD34- cells were sorted based on cell surface markers for purity identification. Afterwards, proliferation and migration of CD34＋ and CD34- cells were detected by MTT assay, colony-formation assay and scratch assay. RESULTS AND CONCLUSION： All four nasopharyngeal carcinoma cell lines expressed CD34 in 0.1%-0.2%, and the level of CD34 was closely related to the cell growth density. The purity of CD34＋ cell was more than 98% in the sorted CD34＋ cell populations, but no CD34＋ cells were found in the sorted CD34- cell populations. At 1, 3, 5 and 7 days the proliferation rate of CD34＋ cell, populations was significantly higher than that of CD34- cells（P 0.05）. Consistently, the colony-formation efficiency of CD34＋ cell was significantly higher than that of CD34- cells（P 0.05）. Moreover, CD34＋ cells migrated significantly faster than CD34- cells by scratch assay（P 0.05）. In conclusion, CD34＋ cells cultured in vitro display higher proliferation and migration capacities, indicating that CD34＋ cells have the potential of nasopharyngeal carcinoma stem cells.

关 键 词：鼻咽肿瘤 肿瘤干细胞 抗原 CD34 组织工程 干细胞 鼻咽癌 肿瘤干细胞标记物 CD34 

分 类 号：R394.2[医药卫生—医学遗传学]