神经嵴细胞发育分子调控网络的生物信息学分析  

The molecular regulatory networks involved in the neural crest cell development:a bioinformatic analysis

作　　者：王君芳 王佳琦[2] 毕庆伟 尹翰文 张国梁[2] 

机构地区：[1]黑龙江省口腔病防治院口腔颌面外科,黑龙江省哈尔滨市150001 [2]佳木斯大学附属口腔医院口腔颌面外科,黑龙江省佳木斯市154002

出　　处：《中国组织工程研究》2016年第24期3621-3627,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:神经嵴细胞发育对中枢神经系统、颅颌面和心脏等组织器官的发生具有重要的作用,受多个基因调控,但各调控基因之间的关系尚不清楚。目的:应用生物信息学方法研究神经嵴细胞发育的分子调控网络。方法:从GEO在线数据库获得在神经嵴细胞发生时差异表达明显的500个基因。通过DAVID和STRING数据库分析这些基因之间的关系。结果与结论:(1)从GEO数据获得的在神经嵴发生时差异表达的500个基经DAVID数据库分析后按照一定的共同属性富集在转化生长因子β信号通路、WNT信号通路、同源盒基因、神经嵴细胞分化、神经管发育等不同的"信号通路"、"分子功能"和"生物学过程"子集;(2)经STRING数据库分析后建立了DLX5、MSX2、SNAIL2、PAX7、SHH、SOX9、NOG、GSC、KAL1、骨形态发生蛋白5、成纤维细胞生长因子8和WNT3a等12个基因的分子网络图,这些基因通过共表达、拮抗、激活等彼此关联;(3)神经嵴细胞发生时许多基因参与了调控,这些基因间存在相互作用并形成网络,提示要从信号通路和分子网络上整体思考神经嵴发育障碍相关疾病的防治。BACKGROUND：Development of neural crest cells,which is regulated by various genes,plays an important role in the formation of central nervous system,heart,craniofacial organs and other tissues.However,the relationship among these genes is unclear.OBJECTIVE：To investigate the molecular regulatory networks involved in the process of neural crest cell development based on a bioinformatic analysis.METHODS：Totally 500 differentially expressed genes during the process of neural crest cell development were obtained from the GEO on-line database.Then the DAVID and STRING on-line databases were used to evaluate the relationship among these genes.RESULTS AND CONCLUSION：Totally 500 differentially expressed genes during the process of neural crest cell development could be enriched into different subgroups based on the analysis of DAVID database,including ＂transforming growth factor β signal pathway＂,＂WNT signal pathway＂,＂homeobox gene＂,＂neural crest cell differentiation＂ and ＂neural tube development＂.Additionally,12 genes molecular networks were built based on the analysis of STRING database,such as DLX5,MSX2,SNAIL2,PAX7,SHH,SOX9,NOG,GSC,KAL1,bone morphogenetic protein 5,fibroblast growth factor 8 and WNT3 a.These genes exhibited interactions by co-expression,activation and antagonism.Therefore,many genes involved in the process of neural crest cell development were interacted and formed the networks.These findings imply that we should understand these neural crest-related diseases from a holistic view of the signaling pathway and molecular regulatory networks.

关 键 词：基因调节网络 基因调节网络 计算生物学 组织构建 组织工程 神经嵴细胞 发育 分子网络 生物信息学分析 

分 类 号：R318[医药卫生—生物医学工程]