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作 者:Fuyun HOU Aixian LI Haiyan ZHANG Shunxu DONG Beitao XIE Liming ZHANG Qingmei WANG
机构地区:[1]Crop Research Institute, Shandong Academy of Agricultural Sciences [2]Scientific Observation and Experimental Station of Potato in Huang-Huai-Hai Region, Ministry of Agriculture
出 处:《Agricultural Science & Technology》2016年第6期1376-1378,1474,共4页农业科学与技术(英文版)
基 金:Support by the Youth Fund of Shandong Academy of Agricultural Science(2014QNM31);the China Agriculture Research System of Sweet Potato(CARS-11-B-06)
摘 要:Anthocyanin accumulation during storage root development in purple-fleshed sweet potato was analyzed by detection of anthocyanin concentration, accumulation rate and the expression pattern of anthocyanin biosynthetic genes by semi-quantitative RT-PCR. Anthocyanin concentration in sweet potato cvs Jishu 18 and Ayamurasaki increased steadily during storage root development stage. The accumulation rate in two genotypes peaked at 50 to 65 d after transplanting, and then declined rapidly. During storage root development of Ayamurasaki, the anthocyanin biosynthesis gene, IbCHS, was constitutively expressed, the genes IbF3H, IbDFR, IbANS were induced steadily, reaching a maximum at the later stage of root thickening, and IbPAL steadily decreased. Therefore, the mechanism of anthocyanin accumulation differed between the two cultivars, and anthocyanin biosynthesis was regulated through regulation of its synthetic enzymes.Anthocyanin accumulation during storage root development in purple-fleshed sweet potato was analyzed by detection of anthocyanin concentration, accumulation rate and the expression pattern of anthocyanin biosynthetic genes by semi-quantitative RT-PCR. Anthocyanin concentration in sweet potato cvs Jishu 18 and Ayamurasaki increased steadily during storage root development stage. The accumulation rate in two genotypes peaked at 50 to 65 d after transplanting, and then declined rapidly. During storage root development of Ayamurasaki, the anthocyanin biosynthesis gene, IbCHS, was constitutively expressed, the genes IbF3H, IbDFR, IbANS were induced steadily, reaching a maximum at the later stage of root thickening, and IbPAL steadily decreased. Therefore, the mechanism of anthocyanin accumulation differed between the two cultivars, and anthocyanin biosynthesis was regulated through regulation of its synthetic enzymes.
关 键 词:Ipomoea Lam anthocyanin sweet potato Sweet purple biosynthesis enzymes steadily
分 类 号:S531[农业科学—作物学] TS202.3[轻工技术与工程—食品科学]
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