氟离子植入猪骨源羟基磷灰石对破骨样细胞生物学行为影响的体外研究  

Effects of fluoride incorporation into porcine hydroxyapatite on biological responses of osteoclast-like cells:An in vitro study

作　　者：罗欣[1,2] 李志鹏[1,2] 黄宝鑫[1,2] 吴夏怡[1,2] 胡铭轩 陈卓凡[1,2] 

机构地区：[1]中山大学光华口腔医学院.附属口腔医院种植科,广东广州510055 [2]广东省口腔医学重点实验室,广东广州510080

出　　处：《临床口腔医学杂志》2016年第6期335-338,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金(81470783;81400550);广东省自然科学基金(2015A030311051;2016A030310173);广东省医学科研基金(B2014116)

摘　　要：目的:研究氟离子植入猪骨源羟基磷灰石对破骨样细胞体外生物学行为的影响。方法:化学浸泡结合高温烧结法制备氟化猪骨源羟基磷灰石,以小鼠单核巨噬细胞株RAW264.7诱导形成破骨样细胞,扫描电镜观察破骨样细胞在材料表面黏附、分化与功能,CCK-8法检测材料上前破骨样细胞的增殖活性,酶活性定量检测细胞内抗酒石酸酸性磷酸酶(Tartrate resistant acid phosphatase,TRAP)活性。结果:破骨样细胞可在氟离子植入猪骨源羟基磷灰石上黏附、分化并发挥吸收功能,材料对前破骨样细胞的增殖活性无明显抑制作用,但可降低破骨样细胞TRAP的表达。结论:氟离子植入猪骨源羟基磷灰石可抑制破骨样细胞的骨吸收作用。Objective：To evaluate the effect of fluoridation of porcine hydroxyapatite on biological responses of osteoclast-like cells. Method：Fluorinated porcine hydroxyapatite（FPHA）was fabricated from porcine bone by chemical immersion and thermal treatment. Osteoclast-like cells was induced from mouse monocyte-macrophage cell line RAW264.7. Osteoclast-like cells attachment,differentiation and function was observed by scanning electron microscope. The viability of osteoclast-like precursors was determined by cell counting kit-8. Tartrate resistant acid phosphatase（TRAP）activity was detected quantitatively by TRAP assay Kit. Result：Osteoclast-like cells could attach,differentiate and participate in materials resorption on PHA and FPHA disks. No significant inhibiting effect was observed in osteoclast-like precursors viability test. However,a decrease of TRAP activity was detected. Conclusion：FPHA could inhibit the bone resorption function of osteoclast-like cells.

关 键 词：氟离子植入 猪骨源羟基磷灰石 破骨样细胞 

分 类 号：Q813.1[生物学—生物工程] R782.1[医药卫生—口腔医学]