婴幼儿血管瘤组织中β2受体的表达  

Expression of beta 2 adrenergic receptor in infantile hemangiomas tissue

作　　者：赵哲媛[1] 李小丹[2] 陈海洲[1] 陈玙炫 

机构地区：[1]郑州大学附属洛阳市中心医院烧伤整形科,471009 [2]华中科技大学附属协和医院整形外科 [3]解放军第一五四中心医院创伤骨科中心

出　　处：《中华医学美学美容杂志》2016年第3期182-184,共3页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的探讨β2受体在婴幼儿血管瘤组织中的表达及其在血管瘤病理演变过程中的作用。方法将48例婴幼儿血管瘤患者分为增殖期组（29例）和消退期组（19例）。采用免疫荧光技术将两组婴幼儿血管瘤组织进行荧光标记。观察两组照片荧光标志物的显示位置，并应用Image—Pro Plus 6．0软件分析比较内皮细胞核及B。受体阳性的平均荧光强度。结果荧光显示，pz受体于婴幼儿血管瘤中广泛表达，并以内皮细胞核荧光表达处为主。增殖期组内皮细胞核的平均荧光强度0.031±0.002显著高于消退期的0.022±0.002，差异有统计学意义（P〈0.05）。增殖期组Bz受体的平均荧光强度0.035±0.003显著高于消退期组的0．028±0．002，差异有统计学意义（P〈0．05）。结论β2受体主要广泛表达于婴幼儿血管瘤内皮细胞。Objective To detect the expression of beta 2 adrenergic receptor in the infantile hemangiomas （IH） tissue and to explore its role in the pathological evolution of infantile hemangiomas as well. Methods 48 cases of infantile hemangioma were divided into two groups. 29 cases were in the proliferating period, while the other 19 cases were in non-proliferating period. By using immunofluo- rescence technology, the endothelial nuclei and beta 2 adrenergic receptor of the IH tissue were marked by fluorescent tags, respectively, in two groups. The location of fluorescent labeling was shown in photos. By using the Image-Pro Plus 6.0 software, we analyzed and compared the average fluorescence intensity of endothelial cell nucleus and beta 2 adrenergic receptors in these two groups. Results Beta 2 adrenergic receptors were widely expressed in IH tissue, especially in endothelial cell nucleus shown in fluorescent images. The average fluorescence intensity of endothelial cell core in proliferating IH group was 0.031 ±0.002, which was much higher than that of fading period IH group （0.022±0.002）. There was significant statistical different （P〈0.05）. The average fluorescence intensity of beta 2 adrenergic receptor in proliferating IH group was 0.035±0.003, which was much higher than that of fading period IH group （0.028 ± 0.002）. There was significantly statistical different be tween the two groups （P〈0.05）. Conclusions Beta 2 receptors are widely expressed in the endothelial cells of infantile hemangioma.

关 键 词：婴幼儿血管瘤 Β2受体 内皮细胞 免疫荧光技术 

分 类 号：R732.2[医药卫生—肿瘤]