环糊精改性Fe_3O_4纳米微球的制备及其负载多柔比星体外释放的研究  

Preparation and in vitro release study of doxorubicin-loaded cyclodextrin modified Fe_3O_4 nanospheres

作　　者：郭秀丽[1] 许光宇 秦丹丹[3] 白雅竹 任秋颖 郑林萍[1] 

机构地区：[1]河南大学药学院,河南开封475004 [2]开封市卫生学校,河南开封475002 [3]河南大学民生学院,河南开封475004

出　　处：《化学研究》2016年第3期364-368,共5页Chemical Research

基　　金：省教育厅科学技术研究重点项目(14A350009);河南大学民生学院大学生创新创业支持计划项目(MSCXCY2015054)

摘　　要：采用柠檬酸钠作为稳定剂,通过超声辅助水相共沉淀法合成了柠檬酸修饰的Fe_3O_4纳米粒(Fe_3O_4@CA),进一步采用真空干燥法制备了β-环糊精包覆的Fe_3O_4纳米微球(Fe_3O_4@β-CD).分别利用X射线粉末衍射仪、傅立叶变换红外光谱仪、透射电子显微镜、热重分析仪等表征手段对其进行了结构和形貌表征.同时,以多柔比星为模型药物,考察了Fe_3O_4@β-CD微球对多柔比星的体外释放行为.结果表明,Fe_3O_4@CA纳米粒子呈球形或类球形,平均流体力学直径为84nm,具有顺磁性,室温下饱和磁化强度为17.5emu·g-1,红外光谱结果表明,β-环糊精成功的包覆在Fe_3O_4@CA表面,Fe_3O_4@β-CD的平均流体力学直径为104nm,室温下饱和磁化强度为15.7emu·g-1.体外释放结果表明,Fe_3O_4@β-CD-DOX载药系统在PBS(pH=7.4)溶液中释放缓慢,12h累积释放率为45.5%.结果表明,环糊精改性的Fe_3O_4纳米微球在体外有明显的缓释效果,有望成为理想的抗肿瘤药物载体.Fe_3O_4@CA nanoparticles were successfully synthesized by ultrasound-assisted coprecipitation method with citric acid as a stabilizer,and Fe_3O_4@β-CD nanospheres were prepared by vacuum drying.Their structure and morphology were characterized by X-ray powder diffraction,fourier-transform infrared spectroscopy,transmission electron microscopy,thermogravimetric analyzer and other characterization methods.At the same time,the in vitro release behavior of Fe_3O_4@β-CD microspheres to doxorubicin was investigated using doxorubicin as a model drug.Results showed that Fe_3O_4@CA nanoparticles were spherical or spherical like,the average hydrodynamic diameter was 84 nm,and the saturation magnetization was 17.5emu·g-1 at room temperature.Fourier-transform infrared spectroscopy results show thatβ-CD and Fe_3O_4@CA were successfully coupled and the average hydrodynamic diameter was 104 nm,magnetic properties test showed that the magnetic microspheres saturation magnetization was15.7emu·g-1.In vitro release studies showed that the release rate of Fe_3O_4@β-CD-DOX drug loading system in PBS（pH=7.4）solution was slow,and the cumulative release rate of 12 h was 45.5%.Results show that cyclodextrin modified Fe_3O_4 microspheres have obvious sustained-release effect in vitro.It is a potential drug carrier for the delivery of antitumor drugs.

关 键 词：FE3O4 Β-环糊精 多柔比星 体外释放 

分 类 号：R944[医药卫生—药剂学]