TNFAIP1对肝癌细胞HepG2细胞增殖及凋亡的影响  

The Role of TNFAIP1 in the Cell Proliferation and Apoptosis to HepG2

作　　者：莫莉桦 刘宁[1] 寻禹 胡翔[1] 何自力[1] 向双林[1] 

机构地区：[1]湖南师范大学生命科学学院蛋白质化学与发育生物学教育部重点实验室,中国长沙410081

出　　处：《湖南师范大学自然科学学报》2016年第3期14-17,共4页Journal of Natural Science of Hunan Normal University

基　　金：国家自然科学基金面上项目(81372157);中国博士后基金面上资助项目(2014M552139);湖南省自然科学基金资助项目(13JJ6037);湖南省教育厅优秀青年基金资助项目(14B114);湖南省教育厅科技计划资助项目(2014RS4008)

摘　　要：为了深入探讨肝癌的发生机制并寻找肝癌的潜在治疗手段,研究了TNFAIP1基因对肝癌细胞HepG2的细胞增殖及细胞凋亡产生的影响.MTT实验发现过表达TNFAIP1基因或干扰TNFAIP1的表达可以显著地抑制或促进HepG2细胞的生长,这表明TNFAIP1基因对肝癌细胞的增殖具有抑制作用.而流式细胞技术则证实过表达TNFAIP1能显著地促进肝癌细胞HepG2的细胞凋亡.To investigate the molecular mechanisms of hepatocellular carcinoma（HCC） progression and develop novel potential therapeutic method for treatment of HCC, in the study, the role of TNFAIP1 in the cell proliferation and apoptosis to HepG2 cells was determined. MTT assay showed that overexpression and knockdown of TN- FAIP1 gene could significantly inhibit and promote cell growth of HepG2 cell lines, respectively. These results imply that TNFA1P1 is involved in the inhibition of cell proliferation in HCC. Moreover, FACS assay indicated that overexpression of TNFAIP1 significantly induced cell apoptosis in HepG2 cell lines.

关 键 词：TNFAIP1 HEPG2 细胞增殖 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]