vIL-10对鼻咽癌细胞株MHC-1类分子抗原加工提呈“操纵子”表达的影响  

Effects of vIL-10 on MHC-I antigen processing“the operon”in nasopharyngeal carcinoma cell lines

作　　者：任艳鑫[1] 杨洁[1] 孙瑞梅[1] 赵留芳[1] 李磊[1] 张世文[1] 费继敏[1] 尚艺泰 李舟蕾 李晓江[1] 

机构地区：[1]昆明医科大学第三附属医院,云南省肿瘤医院头颈外科,云南昆明650118

出　　处：《西安交通大学学报（医学版）》2016年第4期525-530,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：2013年国家自然科学基金(No.81260312);2014年云南省科技厅-昆明医科大学应用基础研究联合专项(No.2014FZ037);2012年云南省卫生科技内设研究机构项目资助(No.2012 WS0035);2014年昆明市西山区科技计划项目(No.西科字50号)~~

摘　　要：目的研究病毒白介素-10(vIL-10)对鼻咽癌细胞株MHC-1类分子抗原加工提呈"操纵子"表达的影响。方法收集不同时间段vIL-10处理的鼻咽癌细胞(CNE-1和CNE-2),采用RT-PCR、Western blot方法检测vIL-10处理的鼻咽癌细胞中MHC-1类分子抗原加工提呈"操纵子"(TAP-1、2,LMP-2、7及HLA-I)表达的变化。结果1mRNA水平:CNE-1和CNE-2的TAP-1水平在不同时间点均未表现出显著差异;vIL-10作用1、4、6、12h时,CNE-1和CNE-2的TAP-2、LMP-2均未出现变化,24h时,两者均出现显著下降甚至消失;CNE-1在vIL-10作用后4h即出现LMP-7下降,而CNE-2在vIL-10作用后6h出现下降;在vIL-10作用24h时CNE-1和CNE-2的HLA-I出现显著下降。2蛋白水平:vIL-10作用后24h时,两种细胞的TAP-1均出现显著下降,TAP-2则在vIL-10作用后1、6、12、24h出现逐渐下降;CNE-2的LMP-2、LMP-7在vIL-10作用后随时间逐渐下降,而CNE-1的两种蛋白仅在作用后12h出现显著下降;HLA-I在vIL-10作用后出现下降趋势,但在CNE-1中各时间段变化无统计学差异,CNE-2在24h显著下降。结论 vIL-10可明显抑制鼻咽癌MHC-I类分子抗原加工和提呈的"操纵子"的表达,并且呈一定的时间依赖性。Objective To explore the effects of virus interleukin‐10 （vIL‐10 ） on different expressions of MHC‐I antigen processing “the operon” .Methods We collected nasopharyngeal carcinoma cells （CNE‐1 and CNE‐2） treated by vIL‐10 at different time points ,and detected the changes of MHC‐I antigen processing “the operons” （TAP‐1 ,TAP‐2 ,LMP‐2 ,LMP‐7 and HLA‐I） by RT‐PCR and Western blot .Results ① mRNA level ：There was no difference in the expression of TAP‐1 in CNE‐1 and CNE‐2 cells at various time points .The expressions of TAP‐2 and LMP‐2 in CNE‐1 and CNE‐2 did not change at 1 ,4 ,6 ,12 h ,but downregulated and even disappeared at 24 h .The expression of LMP‐7 in CNE‐1 decreased 4 h after vIL‐10 was added ,and that in CNE‐2 decreased at 6 h .The expression of HLA‐I in CNE‐1 and CNE‐2 showed significant decrease at 24 h .② Protein expression ：The expression of TAP‐1 in CNE‐1 and CNE‐2 showed significant decrease at 24 h .The expression of TAP‐2 in CNE‐1 and CNE‐2 was gradually downregulated at different time points .The expressions of LMP‐2 and LMP‐7 in CNE‐2 were gradually downregulated at different periods ,while that in CNE‐1 was only decreased at 12 h .The expression of HLA‐I in CNE‐1 and CNE‐2 was gradually downregulated ,but there was no significant difference at each period in CNE‐1 ,while the expression of HLA‐I in CNE‐2 at 24 h was significantly downregulated .Conclusion vIL‐10 can inhibit the expression of MHC‐I antigen processing “the operon” in NPC in the time‐dependent manner .

关 键 词：鼻咽肿瘤 病毒白介素-10 抗原处理相关转运体蛋白(TAP) 低分子量多肽复合物(LMP) 人类白细胞抗原-I(HLA-I) 

分 类 号：R739.63[医药卫生—肿瘤]