microRNA-451在弥漫性大B细胞淋巴瘤患者血浆中的表达及意义  

Expression levels and clinical significance of microRNA-451 in the plasma of patients with diffuse large B lymphoma

作　　者：张平[1] 

机构地区：[1]天津市第五中心医院血液肿瘤内科,天津300450

出　　处：《西安交通大学学报（医学版）》2016年第4期609-612,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81471175)~~

摘　　要：目的探讨microRNA-451(miR-451)在弥漫性大B细胞淋巴瘤(diffuse large B lymphoma,DLBCL)患者血浆中的表达水平及其临床意义。方法利用逆转录聚合酶链反应(reverse transcription polymerase chain reaction,RTPCR)方法检测27例DLBCL患者和27例健康体检者血浆中miR-451的表达水平,并统计分析DLBCL患者血浆中miR-451表达水平与临床病理参数之间的关系。结果 miR-451在DLBCL患者血浆中表达水平明显低于正常对照组[(0.41±0.15)vs.(0.64±0.21)],二者差异具有统计学意义(P<0.01);DLBCL患者血浆中miR-451表达水平在不同性别、年龄、血清LDH水平及原发部位之间差异无统计学意义(P>0.05),而与Ann Arbor分期及IPI评分明显相关(P<0.05)。结论 DLBCL患者血浆中miR-451表达水平降低,可能参与了DLBCL的病理过程。Objective To explore the expression level and clinical significance of microRNA‐451 （miR‐451） in the plasma of patients with diffuse large B lymphoma （DLBCL） .Methods We enrolled 27 patients with DLBCL and 27 healthy controls for the study .The plasma level of miR‐451 was detected by reverse transcription polymerase chain reaction （RT‐PCR） .Then we analyzed the relationship between the plasma level of miR‐451 and pathological characteristics of DLBCL .Results The plasma level of miR‐451 in the patients with DLBCL was significantly lower than in healthy controls [（0 .41 ± 0 .15） vs .（0 .64 ± 0 .21）] （P〈0 .01） .However ,the plasma level of miR‐451 was not significantly correlated with the patients＇gender ,age ,serum LDH level or primary site （P〉0 .05） ,but with Ann Arbor tumor stage and IPI score （ P〈 0 .05 ） .Conclusion The plasma level of miR‐451 decreases in patients with DLBCL ,which indicates that miR‐451 may participate in the pathology of DLBCL .

关 键 词：弥漫性大B细胞淋巴瘤 microRNA-451 定量PCR 

分 类 号：R733.4[医药卫生—肿瘤]