Akt-eNOS信号通路介导去甲肾上腺素调节内皮祖细胞动员  

Akt-eNOS signal pathway for mediating norepinephrine regulating mobilization of endothelial progenitor cells

作　　者：姜其钧[1] 龚志刚[1] 李志刚[1] 丁世芳[1] 

机构地区：[1]广州军区武汉总医院心血管内科,武汉430070

出　　处：《重庆医学》2016年第19期2602-2605,共4页Chongqing medicine

基　　金：湖北省自然科学基金面上资助项目(2014CFC1055);湖北省自然科学基金重点资助项目(2014CAF066)

摘　　要：目的探讨去甲肾上腺素(NE)对小鼠内皮祖细胞(EPCs)增殖能力、迁移能力及从骨髓动员的影响,并分析其分子机制。方法取8周大C57小鼠随机分为3组,各5只,分别为:空白对照组(皮下注射生理盐水,无手术),模型组(皮下注射生理盐水,左下肢肢体缺血),NE组(皮下注射NE 100μmol/100μL,左下肢肢体缺血)。采用小鼠左下肢股动脉结扎术制备肢体缺血模型,微渗泵持续泵入NE,流式细胞仪检测小鼠骨髓、外周血和脾脏中EPCs;培养人外周血EPCs,用NE刺激,检测EPCs的增殖和迁移能力,以及Akt-eNOS信号通路的激活情况。结果 NE能够促进肢体缺血小鼠骨髓EPCs的动员,增加外周血和脾脏的EPCs,NE组与模型组比较,骨髓[(3.271±0.772)%vs.(1.320±0.256)%]、外周血[(0.261±0.041)%vs.(0.110±0.028)%]和脾脏[(4.671±0.345)%vs.(1.880±0.0.381)%]EPCs均增加,差异均有统计学意义(P<0.01)。NE能促进EPCs的增殖和迁移能力,且能够呈浓度依赖性地激活EPCs内的Akt-eNOS信号通路。结论 NE通过Akt-eNOS促进EPCs的迁移和增殖,以及在小鼠骨髓中的动员。Objective To investigate the effect of norepinephrine （NE） on the proliferation and migration capacity of endo- thelial progenitor cells （EPCs） ,and bone marrow mobilization and to analyze its molecular mechanism. Methods The 8-week old C57 mice were taken and randomly divided into 3 groups, 5 eases in each group： the blank control group（subcutaneous injection of normal saline without operaion）, model group（subcutaneous injection of normal saline and ischemia in left lower extremity） and NE group（subcutaneous injection of NE 100 t^mol/100 ~L and ischemia in left lower extremity）. The limb ischemia model was prepared by adopting the femoral arterial ligation in mouse left lower extremity, then NE was continuously pumped by the micro-osmotic pump. The EPCs contents from bone marrow,peripheral blood and spleen were assayed with the flow cytometric analyzer; human peripheral blood EPCs were cultured and stimulated by NE. The proliferation and migration capacity,and the activation situation of Akt and eNOS signal pathway were detected. Results NE could promote the mobilization of bone marrow EPCs in limb ischemia mice, increased the EPCs quantity of peripheral blood and spleen, comparing the NE group with the model group, the EPCs quantity was increased for bone marrow[（3. 271~0. 772）~/00 vs. （1. 320±0. 256）%],peripheral circulation]-（0. 261±0. 041）% vs. （0. 110± 0. 028） %] and spleen[-（4. 671±0. 345）% vs. （1. 880 ±0.0. 381） %], the differences were statistically significant（P〈0.01 ）. NE could promote the proliferation and migration capacity,moreover could activate the Akt-eNOS signal pathway in EPCs with a dose dependent manner. Conclusion NE could promote the proliferation and migration of EPCs and mouse bone marrow mobilization via the Akt-eNOS signal pathway.

关 键 词：内皮祖细胞 去甲肾上腺素 增殖能力 迁移能力 动员 

分 类 号：R363[医药卫生—病理学]