人脐带间充质干细胞移植对压力性尿失禁Wistar大鼠模型尿道括约肌作用的影响  

Effects of human umbilical mesenchymal stem cell on Wistar rats models of stress urinary inconti- nence and its possible mechanism

作　　者：李能[1] 何慧[2] 罗军[1] 苏波[3] 唐爱琼[1] 

机构地区：[1]湖南省妇幼保健院妇科,长沙410008 [2]南华大学解剖学教研室,湖南省衡阳421001 [3]南华大学药物药理研究所,湖南省衡阳421001

出　　处：《中国医师杂志》2016年第6期875-879,883,共6页Journal of Chinese Physician

摘　　要：目的探讨人脐带间充质干细胞（hUCMSCs）移植对压力性尿失禁（SUI）Wistar大鼠模型尿道括约肌收缩功能的影响及其可能机制。方法从足月剖宫产孕妇脐带分离提取hUCMSCs，采用难产产伤及卵巢切除的方法建立SUI大鼠模型，将建模成功并鉴定的SUI大鼠随机分为Model组（10只）、TGF-B1组（10只）、hUCMSCs组（10只），另外取10只正常大鼠作为Normal组；将经TGF—β1和hUCMSCs治疗过的SUI模型大鼠饲养1个月后，检测其膀胱最大容积（MBC）、漏尿点压力（LPP）和腹部漏尿点压力（ALPP）并进行喷嚏实验，然后处死大鼠，取尿道括约肌行HE染色，Westernblot检测其肌钙蛋白（Tn／TnT、TnI、TnC）、原肌球蛋白（Tm）、肌动蛋白（AT）、肌球蛋白重链（MHC）、肌球蛋白轻链（MLC）表达。结果成功提取并鉴定hUCMSCs细胞，成功建造并鉴定Wistar大鼠SUI模型；与Model组和TGF-β1组比较，经hUCMSCs移植治疗的大鼠MBC、LPP和AI胛较模型组显著提高（P〈0．05），而喷嚏实验阳性率显著降低（P〈0．05）；HE染色提示SUI大鼠模型尿道括约肌变细、断裂、稀疏、排列紊乱，伴随着TnI、TnC、TnT、Tm、MHC和MLC蛋白表达减少（P〈0．05），而经hUCMSCs治疗后，尿道括约肌组织结构基本恢复正常，同时TnI、TnC、TnT、Tm、MHC和MLC蛋白表达增加（P〈0．05）。结论hUCMSCs移植能显著改善Wistar大鼠SUI模型临床症状，上调尿道括约肌TnI、TnC、TnT、Tm、MHC和MLC蛋白表达，可能参与了尿道括约肌修复及重建相关机制。Objective To investigate the effects of human umbilical mesenchymal stem cells （hUCMSCs） on Wistar rat models of stress urinary incontinence and its possible mechanism. Methods hUCMSCs ceils were separated and extracted from human umbilical Cord of cesarean delivery of full-term pregnancy women. The models of stress urinary incontinence （SUI） of Wistar rats were established by imitating delivery damage and ovary removing surgery, and then randomly divided into three groups： model group, transforming growth factor-β1 （TGF-β1 ） group, hUCMSCs group, and normal rats as normal group; and every group included ten rats. Maximal bladder capacity （MBC）, leak point pressure （LPP）, abdomi- nal leak point pressure （ALPP）, and sneezing experiment of rats including normal rats, model rats and model rats accepted TGF-β1 or hUCMSCs treatment were examined. When the treatment accomplished, the rats were killed and urethral sphincter were separated and examined by hematoxylin eosin （HE） staining and the proteins of troponin I （TnI）, troponin C （TnC）, troponin T （ TnT）, tropomyosin （ Tm）, actin （AT）, myosin heavy chain （MHC） and myosin light chain （MLC） of urethral sphincter was detected with Western blot method. Results The cells of hUCMSCs were extracted and authenticated, SUI models of Wistar rats were successfully established and authenticated. Compared to model group and TGF-β1 group, the MBC, LPP and ALPP of hUCMSCs group dramatically improved （ P 〈 0. 05）, while the positive rate of sneezing experiment significantly dropped （ P 〈 0. 05）. HE staining showed urethral sphincter became at- tenuation, fracture, sparse and disorder, simultaneously the proteins of TnI, TnC, TnT, Tm, MHC and MLC of urethral sphincter reduced （ P 〈 0. 05 ）. While the damaged urethral sphincter basically restored the normal structure and the proteins of TnI, TnC, TnT, Tm, MHC and MLC of urethral sphincter up-regu- lated through hUCMSCs treatment （ P 〈 0. 05 ）. Concl

关 键 词：脐血干细胞移植 尿失禁 压力性/外科学/病理生理学 尿道/病理生理学 

分 类 号：R-332[医药卫生] R694.54