Selection of Strains Producing Lipase with Transesterification Activity and Its Characterization  

作　　者：Gabriel Luls Castighoni Jorge Alberto Vieira Costa Giovana da Silva Padilha Ranulfo Monte-Alegre 

机构地区：[1]Department of Food Engineering, School of Agronomy, Federal University of Goi6s, Rodovia Goifnia/Nova Veneza, Km O, Goiania, GO 74690-900, Brazil [2]Department of Chemical Engineering, Federal University of Rio Grande, Rua Engenheiro Alfredo Huch, Rio Grande 96201-900, Brazil [3]Department of Food Engineering, University of the State of Campinas, Rua Monteiro Lobato 80, Campinas 13083-862, Brazil

出　　处：《Journal of Chemistry and Chemical Engineering》2016年第2期68-76,共9页化学与化工（英文版）

摘　　要：The interest for lipase production is due to the ability of this enzyme to catalyze some reactions, such as the transesterification. Although industrial biodiesel is produced chemically, there are several problems associated with this technology that can be prevented through the use of lipases. The present work aimed to select microorganisms with potential for production of lipase with transesterification activity. The lipase from Burkholderia cepacia was the one with the most promising results for this type of reaction, showing results of hydrolytic activity at 37 ℃and pH 8.0. The pH and volume of crude enzyme extract that showed favorable for synthesis ofbiodiesel is at about pH 6.0 and 3.75 mL, respectively, which represents approximately 42% of water in the system, ensuring the conversion of nearly 60% to biodiesel.

关 键 词：BIODIESEL Burkholderia cepacia CHARACTERIZATION LIPASE transesterification. 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] TQ426.94