SPF级与普通级FMMU白化豚鼠基础生理生化指标的比较  

The measurement and comparison of basic physiological and biochemical index in SPF and conventional FMMU albimo guinea-pig

作　　者：张晓玉[1] 张修彦[1] 陈沃勋 陈伟盛[1] 梁宁[1] 詹纯列[1] 

机构地区：[1]广州军区广州总医院动物实验中心,广州510010

出　　处：《生物学杂志》2016年第3期47-51,共5页Journal of Biology

基　　金：广东省科技计划科技基础条件建设项目(编号2012B060300022)

摘　　要：为了测定和比较SPF级与普通级FMMU白化豚鼠血液指标、心率、颈总动脉压及心室压、脏器参数,取250~350 g SPF级和普通级FMMU白化豚鼠各30只,雌雄各半,饲养1周后,心脏采血测定血液生理生化及血气指标;颈总动脉插管测定动脉压,呼吸支持情况下开胸进行心室压测定;解剖并精确称量各脏器重量。结果显示SPF级与普通级豚鼠比较：血液指标中,10项血常规指标、14项血生化指标、15项血气指标的差异有显著性意义（P〈0.05或P〈0.01）;心率、颈总动脉收缩压、平均压以及右心室收缩压、舒张压的差异有显著性意义（P〈0.05或P〈0.01）;8项脏器重量差异及8项脏器系数差异有显著性意义（P〈0.05或P〈0.01）。表明不同环境及微生物控制程度下,SPF级FMMU豚鼠与普通级FMMU豚鼠在免疫功能及代谢相关的血液指标存在较大差异,主要的脏器参数、心率、颈动脉血压及右心室压也存在差异。To determine and compare the parameters of blood,heart rate,carotid artery pressure,ventricular pressure and organ indexes between conventional and specific pathogen free（ SPF） FMMU albino guinea-pig,a total of 30 conventional guinea-pig and 30 SPF guinea-pig about 250- 350 g weight were fed for a week,then blood physiological and biochemical indexes; blood gas and carotid artery pressure were determined; the ventricular pressure was measured by arterial catheterization,and heart rate was measured on the respiratory support condition. Finally,weight of guinea-pig body and main organ was weighed. Comparison of SPF and conventional FMMU albimo guinea-pig showed that the difference of 10 blood physiological indexes,14 blood biochemical indexes and 15 blood gas indexes were totally significant（ P 0. 05 or P 0. 01）. The difference of carotid systolic blood pressure and mean blood pressure,right ventricular systolic pressure and diastolic pressure was significant（ P 0. 05 or P 0. 01）. And there were 8 organs＇ weight and 8 organ coefficients which were significantly different（ P 0. 05 or P 0. 01）. In the different environment and microbial control,there were significant differences in blood indexes related immune function and metabolism between SPF and conventional FMMU albimo guinea pigs. The main organ parameters,heart rate,blood pressure and right ventricular pressure were also different.

关 键 词：FMMU白化豚鼠 血液指标 颈总动脉压 心室压 脏器参数 

分 类 号：R-332[医药卫生]