阻断巨噬细胞集落刺激因子抑制子宫内膜异位症基质细胞迁移研究  

Study on the knockdown of macrophage colony-stimulating factor in inhibiting migration of endometriosis stromal cells

作　　者：石瑾秋[1] 卢健翔[1] 常亮[2] 李宝艳[1] 

机构地区：[1]深圳市罗湖区人民医院妇科微创治疗中心,广东深圳518001 [2]北京大学第三医院妇产科,北京100191

出　　处：《中国实用妇科与产科杂志》2016年第7期685-688,共4页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：深圳市科技研发资金项目(JCYJ20130402161226917)

摘　　要：目的探讨采用RNA干扰(RNA interference,RNAi)技术干扰巨噬细胞集落刺激因子(macrophage colonystimulating factor,M-CSF)抑制子宫内膜异位症(endometriosis,EMs)基质细胞迁移的可行性,并阐明其分子机制。方法异位子宫内膜组织取自深圳市罗湖区人民医院妇科微创治疗中心2014年1月至6月就诊的患者。取组织后分离培养EMs基质细胞,采用小干扰RNA(small interfering RNA,si RNA)敲低M-CSF表达,通过划痕实验检测EMs基质细胞的迁移能力,通过明胶酶谱及实时荧光定量PCR检测基质金属蛋白酶2,9(matrix metalloproteinase,MMP-2,9)的活性及表达。结果敲低M-CSF后显著抑制EMs基质细胞迁移,且抑制该细胞MMP-2及MMP-9的表达及活性。结论采用RNAi技术敲低M-CSF表达很可能是治疗EMs的新方法。Objective To investigate whether knockdown of macrophage colony-stimulating factor （M-CSF） in endometriosis stromal cells inhibits migration and explore its molecular mechanism. Methods The endometriosis stromal cells were separated from endometriosis patients in the People＇ s Hospital of Luohu District from January to June, 2014, The endometriosis stromal cells were cultured and the expression of M-CSF was knocked down by using specific siRNA. The migration activity of EMs stromal cells was measured by wound-healing assays. The activity of matrix metalloproteinase- 2 and 9 （MMP-2 and 9） was determined by using gelatin zymography. The expression of MMP-2 and MMP-9 was measured with real-time qPCR assay.Results Knockdown of M-CSF in EMs stromal cells inhibits the migration activity and suppresses the activation and expression of MMP-2 and MMP-9.Conclusion Knockdown of M-CSF by using RNAi assays may be a novel therapy for the treatment of EMs.

关 键 词：子宫内膜异位症 巨噬细胞集落刺激因子 迁移 

分 类 号：R711.71[医药卫生—妇产科学]