人白细胞介素-33在胶质瘤中高表达并能促进胶质瘤细胞U251的侵袭作用  

IL-33 expression is increased in human glioma and promotes U251 glioma cells invasion

作　　者：章建飞 曹文达[1] 汪璟[1] 王清[1] 鲁晓杰[1] 

机构地区：[1]南京医科大学附属无锡二院神经外科,江苏无锡214002

出　　处：《南京医科大学学报（自然科学版）》2016年第6期682-686,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：无锡市医管中心重大项目(YGZXZ1406)

摘　　要：目的 :探讨人白细胞介素(interleukin,IL)-33在胶质瘤中的表达及在胶质瘤细胞U251侵袭中的作用。方法 :采用免疫组化法检测了16例正常脑组织及86例不同级别胶质瘤标本IL-33蛋白的表达情况;应用Transwell小室检测IL-33对U251侵袭的影响;应用Western blot检测IL-33对胶质瘤细胞NF-κB及其磷酸化状态的影响。结果:免疫组化结果显示,IL-33在胶质瘤组织中表达高于正常脑组织(54.65%vs.6.25%,P<0.001)。IL-33在低级别胶质瘤(WHOⅠ、Ⅱ级)和高级别胶质瘤(WHOⅢ、Ⅳ级)组织中的阳性表达率分别为17.4%和68.3%(P<0.001)。体外胶质瘤细胞的功能试验中,IL-33能促进U251细胞的侵袭(P<0.05),并伴随NF-κB蛋白表达上调(P<0.05)。结论:IL-33在胶质瘤中高表达,IL-33可能通过促进胶质瘤细胞NF-κB磷酸化,从而促进胶质瘤细胞的侵袭能力,对IL-33作用机制的进一步研究将为胶质瘤的临床治疗提供新思路。Objective：To detect the expression of human interleukin-33 （IL-33） in glioma and study its effects on invasion of human U251 glioma cells. Methods：Immunohistochemistry was performed to detect IL-33 protein expression in 16 normal brain tissues and 86 patients with different grades of gliomas. Transwell assay was conducted to study the effect of IL-33 on invasion of U251 glioma cells. Western blotting assays were performed to investigate changes in NF-κB expression and its phosphorylation status after IL-33 treatment. Results：IL-33 protein expression was significantly higher in glioma tissues compared with normal brain tissues （54.65% vs 6.25%,P 〈 0.001）. The expression of IL-33 in low-grade （WHO Ⅰ and Ⅱ） glioma and high-grade（WHO Ⅲ and Ⅳ） glioma was 17.4% and 68.3% （P 〈 0.001）,respectively. IL-33 enhanced U251 cellular invasion （P 〈 0.05） in vitro,and the protein expression of NF-κB was increased （P 〈 0.05）. Conclusion：IL-33 is frequently overexpressed in glioma tissues,and may contribute to glioma cell invasion by up-regulation of NF-κB phosphorylation.

关 键 词：人白细胞介素-33 胶质瘤细胞 侵袭 

分 类 号：R73-37[医药卫生—肿瘤]