草乌多糖金属配合物的制备、表征与抗癌活性研究  

Preparation,Characterization,and Anticancer Evaluation of Inner Mongolia Radix Aconiti Kusenezoffii Polysaccharide Metal Complexes

作　　者：张茜[1] 芮瑞 李佩佩[1] 冯翠宁 雷强[1] 龙跃[1] 

机构地区：[1]郑州大学化学与分子工程学院,河南郑州450001 [2]锡林郭勒盟蒙医医院内窥镜科,内蒙古锡林浩特026000

出　　处：《郑州大学学报（工学版）》2016年第3期36-39,43,共5页Journal of Zhengzhou University（Engineering Science）

基　　金：河南省科技攻关计划资助项目(0624420031);河南省基础研究基金资助项目(022463001)

摘　　要：以水为提取溶剂从草乌中提取草乌多糖(RPS),确定了多糖提取的最佳工艺条件:提取温度90℃;提取时间4 h;料液比1∶50,并以草乌多糖为配体,制备了4种多糖金属配合物(RPS-Ca、RPS-Zn、RPS-Cu、RPS-Fe).采用MTT(噻唑蓝)法,以配体草乌多糖为对照,测定了4种多糖金属配合物对肝癌细胞(Hep G2)、乳腺癌细胞(MCF-7)和结肠癌细胞(HT-29)的抑制作用.实验结果显示,草乌多糖铜配合物对3种癌细胞表现出最强的抑制作用.草乌多糖铜配合物的结构经紫外光谱(UV)、红外光谱(IR)、圆二色谱(CD)、电镜扫描(SEM)和热重分析(TGA)得到表征.By using water as extraction reagent,the polysaccharides in Radix Aconiti Kusnezoffii were extrac- ted. The results showed that optimum extraction condition was 90℃, 4 h, and liquid to material ratio 1 ： 50. Four polysaccharide complexes were prepared using four metal ions （ Ca2 ＋ , Zn2＋ , Cu＋ , Fe3 ＋ ） and polysaccha- rides from Radix Aconiti Kusnezoffii, respectively. All complexes were evaluated for biological activity against three human cancer cell lines （HepG2, MCF-7 and HT-29） by the standard MTT method, and the results showed that PRS-Cu possessed the highest growth inhibitory effects. The complex was characterized by UV, IR, CD, SEM, and TGA.

关 键 词：草乌 多糖金属配合物 制备 表征 抗癌活性 

分 类 号：O629.1[理学—有机化学]