HDAC2 siRNA转染对卵巢癌ovcar3细胞中p53和乙酰化p53k320表达的影响  

Effect of HDAC2 siRNA transfection on expression of p53 and acetylated p53k320 in ovarian cancer ovcar3 cells

作　　者：张敏[1] 赵书君[1] 朱海[1] 胡晓静[1] 余亚南[1] 韩会会[1] 曹蒙[1] 李红雨[1] 

机构地区：[1]郑州大学第三附属医院妇产科,郑州450052

出　　处：《郑州大学学报（医学版）》2016年第3期372-375,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：郑州市科学技术局2015普通科研项目153PKJGG165

摘　　要：目的:探讨靶向沉默组蛋白去乙酰化酶2(HDAC2)对人卵巢癌ovcar3细胞中p53乙酰化位点k320表达的影响。方法:将ovcar3细胞随机分为空白组、对照组和实验组3组,实验组以HDAC2 siRNA转染细胞,对照组转染对照siRNA,空白组不处理,分别采用实时荧光定量PCR和Western blot方法检测3组细胞中HDAC2 mRNA和蛋白的表达,Western blot检测3组细胞中p53、乙酰化p53k320蛋白的表达。结果:与空白组及对照组相比,实验组HDAC2 mRNA和蛋白的表达降低(P<0.05),p53蛋白和乙酰化p53k320蛋白表达均升高(P<0.05)。结论:下调HDAC2的表达可使p53和乙酰化p53k320表达增加。Aim： To investigate the effect of targeted silencing histone deacetylase 2（ HDAC2） on expression of p53 and acetylation p53k320 in human ovarian cancer cell line ovcar3. Methods： ovcar3 cells were divided into 3 groups.HDAC2 siRNA was used to transfected into the ovcar3 cells in experimental group,and the cells transfected with control siRNA（ control siRNA group） and the cells without treatment（ control group） were the control. Real Time-PCR and Western blot was used to detect the expression of HDAC2 mRNA and protein; the expression of p53 and acethylased p53k320 were examined by Western blot. Results： Compared with control group and control siRNA group,the expressions of HDAC2 mRNA and protein in experimental group were decreased（ P〈0. 05）,and the expressions of p53 and acethylased p53k320 protein were increased（ P〈0. 05）. Conclusion： Downregulating the expression of HDAC2 could increase the expressions of p53 and acethylased p53k320.

关 键 词：组蛋白去乙酰化酶2 P53 乙酰化p53k320 ovcar3细胞株 卵巢肿瘤 

分 类 号：R737.31[医药卫生—肿瘤]