紫外灭活CVB3病毒诱导BALB/c小鼠产生免疫保护作用的研究  

Study of Protective Immune Effect Induced by UV-inactivated CVB3 on the BALB/c Mice

作　　者：何峰[1] 肖宗慧[1] 刘卓[2] 肖萍[3] 姚海兰[1] 冯淼[1] 刘哲伟[1] 

机构地区：[1]首都儿科研究所分子免疫室,北京100020 [2]首都儿科研究所生化室,北京100020 [3]首都儿科研究所附属儿童医院病理科,北京100020

出　　处：《现代生物医学进展》2016年第20期3801-3805,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81271809);北京市自然科学基金项目(7164241)

摘　　要：目的:探讨紫外灭活型CVB3病毒诱导BALB/c小鼠产生特异性免疫应答及保护作用的评估。方法:采用紫外灭活的方法处理野生型CVB3m株,按照0.1 LD50(10~4 PFU)的剂量免疫小鼠,设置PBS免疫组作为对照,免疫后第3,5,7天收集小鼠血清,检测细胞因子含量;在第3,5,7,14天分离小鼠脾脏,流式分析T细胞亚群的分布比例;在免疫后一个月分离小鼠血清,检测中和抗体的滴度;同时间给予小鼠100LD 50野生型CVB3感染,观察小鼠的死亡率。结果:与对照组相比,在检测日期内紫外灭活型CVB3组小鼠血清中细胞因子IL-1α,TNF-α,IL-6的表达量明显增高(P<0.05),IL-4的表达量没有明显差异;免疫后第14天CD3^+CD4^+T细胞的分布较对照组明显升高(P<0.05);在免疫后一个月,紫外灭活型CVB3免疫组可以诱导机体产生高滴度中和抗体,同时,小鼠应对高致死量CVB3感染时有较高的存活率。结论:紫外灭活型CVB3感染能诱导机体产生特异性免疫应答,同时,产生的中和抗体可以提高小鼠应对致死剂量CVB3感染时的生存率,对机体有明显的保护作用。Objective： To investigate specific immune response and protective effect of BALB/c mice induced by UV inactivated CVB3. Methods： Wild CVB3 m strain was inactivated by UV, and male BALB/c mice were immunized with 0.1 LD50 （10^4 PFU） of UV inactivated CVB3, named CVB3-UV, PBS treated mice were set as compared group. On the 3, 5, 7 days post immunization mice were sacrificed, serum were collected and cytokines were detected by CBA method, T cell subset were analyzed by flow cytometry method in the splenocytes on the 3, 5, 7, 14 days post immunization, neutralizing antibody in the sera of immunized mice 1 month later were detected, further, mice survival rate were also evaluated after challenge with lethal dose of wild type CVB3. Results： Compared with PBS treated group, CVB3-UV could induce high levels of IL-1α, TNF-α and IL-6 （P〈0.05）, while IL-4 production was comparable between both groups. CD3^＋CD4^＋T cell development were found affected on the 14 days post immunization by CVB3-UV （P〈0.05）, and higher titer neutralizing antibody were induced in the CVB3-UV treated mice. Importantly, higher survival rate of mice were found in the CVB3-UV group after re-infection by lethal CVB3. Conclusion： UV-inactivated CVB3 could induce specific immune response and support obvious protective effect in BALB/c mice.

关 键 词：紫外灭活型CVB3 细胞因子 中和抗体 免疫保护作用 

分 类 号：R-33[医药卫生]