响应面试验优化果胶酶辅助提取锁阳原花青素工艺  

Optimization of Pectinase-Assisted Extraction of Proanthocyanidin from Cynomorium songaricum Rupr. Using Response Surface Methodology

作　　者：崔玮[1] 张勇[1,2] 高海宁 焦杨[1] 李彩霞[1,2] 

机构地区：[1]河西学院农业与生物技术学院,甘肃张掖734000 [2]甘肃省高校河西走廊特色资源利用省级重点实验室,甘肃张掖734000

出　　处：《食品科学》2016年第14期18-23,共6页Food Science

基　　金：国家中医药管理局2012年中医药行业科研专项(201207002)

摘　　要：利用单因素试验和响应面法优化果胶酶辅助乙醇提取锁阳原花青素工艺。通过单因素试验筛选出酶解时间、p H值、酶解温度、乙醇体积分数作为影响因素,以锁阳原花青素提取得率为响应值进行Box-Behnken试验设计,建立锁阳原花青素提取得率的二次回归方程,得到最优提取条件。响应面法分析结果表明锁阳原花青素的最佳提取工艺参数为:在果胶酶质量分数为1%时,酶解时间34 min、p H 4.8、酶解温度52℃、用体积分数70%乙醇溶液浸提1.5 h。该条件下,锁阳原花青素提取率达14.30%。Purpose： To optimize the pectinase-assisted extraction of proanthocyanidins（PC） from the stems of Cynomorium songaricum Rupr.. Methods： Pectinase was introduced into the process for improving the extraction efficiency. The extraction conditions were optimized by using response surface methodology（RSM）. Five key factors including enzyme dosage, hydrolysis duration, p H value, temperature and ethanol concentration were selected by one-factor-at-a-time experiments. The extraction yield of PC was used as the response. Results： A quadric regression equation for predicting the extraction yield of PC was established using Box-Behnken design and the extraction conditions were optimized as follows： hydrolysis for 34 min with 1% pectinase at 52 ℃ and initial p H 4.8 followed by extraction for 1.5 h with 70% ethanol as the extraction solvent. Under these optimized conditions, the extraction yield of PC reached 14.30%. Conclusion： The optimized pectinase-assisted extraction method can enhance the extraction efficiency of PC from Cynomorium songaricum Rupr..

关 键 词：锁阳 果胶酶 原花青素 响应面法 

分 类 号：TQ461[化学工程—制药化工]