高效液相色谱法测定气血宁口服液中阿魏酸含量  

Content Determination of Ferulic Acid in Qixuening Oral Liquid by HPLC

作　　者：林晓春[1] 翁立冬[2] 郑锦坤[1] 李旭桂[1] 黄升昌 林晓佳[2] 

机构地区：[1]广东省粤北人民医院,广东韶关512026 [2]南方医科大学,广东广州510515

出　　处：《中国药业》2016年第13期71-73,共3页China Pharmaceuticals

基　　金：广东省建设中医药强省科研课题;项目编号:20142143;广东医院药学研究基金;项目编号:2015A32;广东省韶关市科学计划;项目编号:2014CX/K204;广东省韶关市医学科研基金;项目编号:Y14064

摘　　要：目的建立测定气血宁口服液中阿魏酸含量的高效液相色谱（HPLC）法。方法色谱柱采用Kromasil HC-C18柱（250 mm×4.6 mm,5μm）,流动相为乙腈-0.1%磷酸溶液（17∶83）,流速为1.0 m L/min,检测波长为316 nm,柱温为20℃。结果阿魏酸进样量在0.02~0.10μg范围内与峰面积呈现良好的线性关系（r=0.999 9）;平均加样回收率为99.64%,RSD为1.04%（n=6）;阿魏酸含量检测平均结果为0.014 4 g/L。结论该法操作简便、结果准确、重复性良好,可作为气血宁口服液中阿魏酸含量控制方法。Objective To establish an HPLC method for the content determination of ferulic acid in Qixuening Oral Liquid. Methods The Kromasil HC-C18 column （ 250 mm × 4. 6 mm, 5 μm ） was used, acetonitrile -0. 1% phosphoric acid solution （ 17 ：83 ） was used as the mobile phase, the low rate was 1. 0 mL/min and detected at a wavelength of 230 nm, and the temperature was 20 ℃. Results The content of ferulic acid was linear in the range of 0. 02-0. 10 μg （ r=0. 999 9 ） , the average recovery was 99. 64% and RSD was 1. 04%（ n=6）. The average results of ferulic acid content were 0. 014 4 g/L. Conclusion This method is simple, quick and accurate, and can be used for the content determination of ferulic acid in Qixuening Oral Liquid.

关 键 词：气血宁口服液 高效液相色谱法 阿魏酸 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]