Fmr1基因敲除小鼠悬尾实验的观察  

Observation of tail suspension test in Fmr1 gene knockout mice

作　　者：胡丽婵[1] 郭艺[2] 孙祺章[1] 余国汉[1] 黄月玲[3] 戴丽军[3] 党亚梅[1] 黄雄[1] 陈盛强[2] 黄海樱[2] 

机构地区：[1]广州医科大学附属脑科医院,广州市惠爱医院,广州510370 [2]广州医科大学附属第二医院,广州510260 [3]广州医科大学实验动物中心,广州510182

出　　处：《广州医药》2016年第4期9-12,共4页Guangzhou Medical Journal

基　　金：广东省科技计划项目(2013B021800075;2015A0030302090);广州市科技计划项目(2014Y2-00046);广东省中医药局项目(B1533015);广东市卫生局科技项目(20141A10020;20151A011080)

摘　　要：目的对不同周龄的KO小鼠与WT小鼠进行悬尾实验进行观察,探讨KO小鼠与WT小鼠的行为差别。方法采用健康的试验动物180只分两组:1KO组(4、6、8周龄,各周龄30只,雌雄各半,共90只)2WT组(4、6、8周龄,各周龄30只,雌雄各半,共90只);通过悬尾实验观察性别,年龄对不动时间的影响。结果同龄KO雌性小鼠比雄性小鼠的静止时间差别不大;随着年龄增大,静止时间增长。同龄同性别的KO鼠比WT鼠的不动时间长。P<0.05;同龄雄性小鼠比雌性小鼠的不动时间短;随年龄增长各种系小鼠不动时间增长,KO鼠的不动时间比WT鼠长,P<0.05。结论 KO小鼠存在抑郁行为表型。Objective To observe tail suspension test in Fmr1 gene knockout mice and to explore whether there are differences in mobility of KO and WT mice. Methods 1 80 test mice were divided into two groups：① KO group （4,6,8 weeks old,each age group of mice is 30,male and female in half,a total of 90）② WT group （4,6,8 weeks old,each group of mice is 30,male and female on half,a total of 90）.Through forced swimming test and tail suspension test to observe gender, age effect on immobility time. Results With the same age of the same sex,the KO mice’s immobility time was longer than WT mice’s.P 〈0.05.With the same age,the male mice’s immobility time was shorter than female mice’s.With the age in-crease,the immobility time of KO mice was longer than WT mice.P 〈0.05. Conclusion Fmr1 gene knockout mice have anxiety and depressive behavior.

关 键 词：悬尾实验 脆性X综合征 抑郁行为 

分 类 号：R596[医药卫生—内科学]