活性氧在黄孢原毛平革菌产木质素过氧化物酶中的调控机制  

Regulation mechanism of reactive oxygen species in the lignin peroxidase production of Phanerochaete chrysosporium

作　　者：施雅青[1] 郑耀通[1] 

机构地区：[1]福建农林大学资源与环境学院,福建福州350002

出　　处：《江苏农业学报》2016年第3期514-520,共7页Jiangsu Journal of Agricultural Sciences

摘　　要：为了探究活性氧对黄孢原毛平革菌产木质素过氧化物酶的调控机制,以黄孢原毛平革菌(Phanerochaete chrysosporium)野生菌株pc530及突变菌株pcR5305为研究对象,研究两菌株在富氮液体培养基及外加不同浓度活性氧供体条件下,羟自由基(·OH)浓度与木质素过氧化物酶(Li P)活性的动态变化规律。结果表明,两菌株均能产生较高浓度的·OH,且·OH浓度与Li P活性变化趋势相一致,但·OH的绝对浓度与Li P的产生量并不相关;不同浓度的H2O2均能提高两菌株Li P的产生量;外加二甲亚砜(DMSO)和芬顿试剂(Fenton)均能对两供试菌株合成Li P及产生·OH起作用。因此推测,·OH不直接参与或影响两菌株合成Li P,更可能是作为一种信号分子在起作用。活性氧作为一种胁迫因子对两菌株的产酶具有极为重要的作用。Phanerochaete chrysosporium wild type pc530 and mutant strain pcR5305 were cultured to study the dynamic changes of hydroxyl radical（ ·OH） concentration and lignin peroxidase（ Li P） activities in nitrogen-rich liquid medium and in the medium added with various concentrations of reactive oxygen species（ ROS） donor（ H2O2）. Both strains produced high concentrations of ·OH,and the change of ·OH concentration was similar to that of Li P. No correlation was found between the absolute concentration of ·OH and the Li P yield. The yield of Li P produced by the two strains was enhanced by H2O2 despite of concentration. Additional DMSO and Fenton influenced Li P synthesis and ·OH production for both strains. The results suggest that ·OH is likely to act as a signalling molecule instead of being directly involved in the synthesis of the Li P,and active oxygen as a stress factor plays an important role in enzyme production by the two strains.

关 键 词：活性氧 黄孢原毛平革菌 木质素过氧化物酶 调控机制 

分 类 号：X172[环境科学与工程—环境科学]