银菘香的急性毒性及致突变性研究  

Research on the Acute and Mutagenic Toxic Effects of Yinsongxiang Compound

作　　者：吴彦[1] 邸莎[2] 李贤煜[3] 孔冉[2] 安婉丽 李志华[1] 刘绍华[1] 黄东业[1] 田兆福[1] 杨鸿[3] 

机构地区：[1]广西中烟工业有限责任公司技术中心,广西南宁530001 [2]北京中医药大学,北京100029 [3]中国中医科学院医学实验中心,北京100700

出　　处：《中国药物警戒》2016年第6期321-324,337,共5页Chinese Journal of Pharmacovigilance

基　　金：广西壮族自治区技术创新项目(桂工信科技[2014]1012号);广西科学研究与技术开发计划课题(桂科攻15118004)

摘　　要：目的在前期"银菘香"中药复方的抗病毒抗菌的研究结果基础上,对由中药板蓝根、淡竹叶、菊花、金银花提取液和甘草浸膏配伍而成的复方进行了急性毒性和致突变性研究,为其临床应用提供理论依据。方法采用小鼠急性经口毒性试验、小鼠骨髓嗜多染红细胞微核试验、小鼠骨髓细胞染色体畸变试验和Ames试验4种方法。结果急性毒性试验显示,雌、雄小鼠经口给药最大耐受量(MTD)大于15 g·kg-1体重,结果为实际无毒。小鼠骨髓嗜多染红细胞微核试验、小鼠骨髓细胞染色体畸变试验和Ames试验显示,该药致突变实验结果均为阴性,显示该复方未见致突变性影响。结论中药复方"银菘香"未见急性毒性和致突变影响,可见该药物安全性良好。Objective To evaluate the acute and mutagenic toxic effects of Yinsongxiang compound. Chinese herbal active ingredient formula of Yinsongxiang compound was composed ofIsatidisRadix, Lophatheri Herba,Chrysanthemi Flos,Lonicevae japonicae Flosextract andGlycyrrhizae Radix et Rhizomaextract.Methods Acute oral toxicity test, marrow cell micronucleus test（PCE）, marrow chromosomal aberration test of mice and Ames were tested.Results Mice acute oral MTD of the formula was higher than 15 g·kg-1, and the micronucleus rates in all doses were not significantly different from the control group. Negative results were seen from chromosome aberration test, and no increase in the number of revertant colonies was found in the Ames test with and without S9 activation system.Conclusion The Chinese herbal active ingredient formula has no toxic substance and mutagenicity effects in this study.

关 键 词：中药复方 急性毒性 致突变性 微核 染色体畸变试验 AMES试验 

分 类 号：R965.3[医药卫生—药理学]