丙戊酸对颅脑损伤大鼠海马成体神经干细胞原位激活的作用  

Effect of valproic acid on the situ activation of hippocampal adult neural stem cells in brain tissue of rats with experimental traumatic brain injury

作　　者：杨伟锋[1] 金保哲[2] 张新中[2] 王磊[1] 周文科[2] 王仲伟[2] 

机构地区：[1]新乡医学院,河南新乡453003 [2]新乡医学院第一附属医院神经外科,河南卫辉453100

出　　处：《新乡医学院学报》2016年第6期469-472,共4页Journal of Xinxiang Medical University

基　　金：河南省医学科技攻关计划项目(编号:201203069);河南省教育厅自然科学研究计划资助项目(编号:12A320012)

摘　　要：目的观察丙戊酸(VPA)对颅脑损伤后大鼠海马成体神经干细胞(NSC)原位激活的作用。方法将成年健康Sprague-Dawley雄性大鼠66只随机分为正常对照组(n=6)、单纯损伤组(n=30)和VPA治疗组(n=30),正常对照组大鼠不做任何处理,单纯损伤组及VPA治疗组大鼠建立闭合性颅脑损伤模型;VPA治疗组大鼠按每天300 mg·kg-1的剂量经腹腔注射给药进行干预,单纯损伤组大鼠给予腹腔注射等量的生理盐水进行干预,分别于颅脑损伤后3、7、14、21、28 d断头取脑标本,采用免疫荧光技术检测各组大鼠不同时间点海马齿状回内5-乙炔基-2'-脱氧尿嘧啶核苷(Edu)阳性细胞数及大鼠海马齿状回内巢蛋白(Nestin)阳性细胞数的变化。结果单纯损伤组和VPA治疗组大鼠海马齿状回内Edu阳性细胞数及Nestin阳性细胞数于造模后第3天开始增高,于造模后第21天达高峰,在同一时间点单纯损伤组和VPA治疗组大鼠Edu阳性细胞数及Nestin阳性细胞数均高于正常对照组(P<0.01),而VPA治疗组各时间点Edu阳性细胞数及Nestin阳性细胞数较单纯损伤组明显增加(P<0.01)。结论丙戊酸对颅脑损伤大鼠海马成体NSC有原位激活作用。Objective To observe the effect of valproic acid（ VPA） on the situ activation of hippocampal adut neural stem cells（ NSC） in brain tissue of rats with experimental traumatic brain injury. Methods Sixty-six healthy male SpragueDawley rats were randomly divided into normal control group（ n = 6）,simple trauma group（ n = 30） and VPA treated group（ n = 30）. The rats in normal group were not given any thing. Rat model of closed head injury was established in simple trauma group and VPA treated group. The VPA treated group was interfered by intraperitoneal injecting VPA according to the daily dose of 300 mg·kg^-1·d^-1; the simple trauma group was intraperitoneal injected the same amount of saline to intervene. All the rats were sacrificed by decapitation at 3,7,14,21,28 days after brain injury. Immunofluorescence was employed to detect the changes of 5-ethynyl-2＇- deoxyuridine（ Edu） positive cells and nest protein（ Nestin） in hippocampal dentate gyrus of each rats. Results The Edu and Nestin positive cells number in the hippocampal dentate gyrus of simple trauma group and VPA treated group were rised within 3 days after the damage,and were rised to a peak within 21 days after the damage. At the same time,the number of Edu and Nestin positive cells of the simple trauma group and VPA treated group rats were higher than those of the normal control group; at each time point,the number of Edu and Nestin positive cells increased more significantly in the VPA treated group compared with simple trauma group（ P〈0. 01）. Conclusion VPA has situ activation on hippocampal adult NSC in brain tissue of rats with experimental traumatic brain injury.

关 键 词：创伤性颅脑损伤 丙戊酸 大鼠 神经干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]