醋酸质量对CE-MS测定蛋白质肽图谱图质量的影响  

Effect of Acetic Acid Quality for Determination of Protein Peptide Mapping by Capillary Electrophoresis-Mass Spectrometry

作　　者：李晓[1] 徐延昭[1] 

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《现代科学仪器》2016年第3期68-71,76,共5页Modern Scientific Instruments

摘　　要：摘要目的：考察醋酸质量对CE-MS结果的影响。方法：在使用不同级别的醋酸条件下，进口高质量分析纯和LC--MS级别醋酸，采用CE—Ms分析蛋白质质量肽图。结论：本文对比了进口高质量分析纯醋酸和LC—MS级别醋酸在CE—Ms分析蛋白质肽图时的影响。在空白实验中，LC—Ms级醋酸的TIE波动程度约为分析纯醋酸的一半；对比谱图基线发现，采用LC—MS级别醋酸在m／z=400处基线高度仅为基峰的5％，但是如果采用分析纯醋酸，该处基线高度可达基峰的20％，基线噪音提高4倍；在BSA实验组中，分析纯醋酸组灵敏度为采用LC-MS级别醋酸的1／2-1／5。因此，LC-MS级试剂是毛细管电泳质谱联用技术的首选。Objective： Studying the effect of acetic acid quality of CE-MS results. Methods： Under the condition of the use of different levels of acetic acid, high quality analytically pure level and LC-MS level acetic acid were imported, the protein peptide mapping was analyzed by CE-MS. Conclusion： in this article, we compared the influence of the imported high quality analytically pure level and LC-MS level acetic acid in the CE-MS analysis protein peptide mapping. In blank experiment, TIE volatility of LC-MS level acetic acid is about half of the analytically pure acetic acid. Comparingthe chromatogram baseline, the LC-MS level of acetic acid in the m/z=400 baseline height was only about 5% of the base peak, but if pure acetic acid is analyzed, the baseline height can be up to 20% of the base peak, four times baseline noise increases. In the B SA experimental group, the sensitivity of the analytically pure group was 1/2-1/3 of the LC - MS group So LC - MS level reagent is the first selection of CE-MS technology

关 键 词：毛细管电泳质谱联用 醋酸质量 灵敏度 

分 类 号：O657.63[理学—分析化学]