气质联用-内标法测定红花籽中脂肪酸含量  

Determination of Fatty Acids from Safflower Seeds by Gas Chromatography Mass Spectrometry Using Internal Standard Method

作　　者：杨莲[1] 钱宗耀[1] 王建梅[1] 

机构地区：[1]新疆农业科学院农业质量标准与检测技术研究所,新疆乌鲁木齐850091

出　　处：《现代科学仪器》2016年第3期77-79,83,共4页Modern Scientific Instruments

摘　　要：摘要对新疆无公害农产品红花种植基地的红花籽脂肪酸化合物成分进行含量测定研究。通过氢氧化钾一甲酯酯化反应，以十一烷酸甲酯为内标物，正己烷为溶剂，采用气相色谱一质谱联用技术对脂肪酸甲酯成分进行分离并辅助NIST检索工具分析成分。建立内标法计算红花籽中脂肪酸含量的方法。结果表明，红花籽中不饱和脂肪酸亚油酸含量接近20％（w／w）。该实验方法简便快捷，可为内标法测定脂肪酸含量研究提供借鉴。The present study focus on the composition of fatty acids in commercially available safflower seed in Xinjiang production area. Through esterification reaction, the hexane was used as solvent direct injection and quantified by internal standard C l1：0 methyl ester. Gas chromatography-mass spectrometry was used for analysis of fatty acid methyl ester, and the NIST library search was assisted to identify various components. The results showed that safflower seed is mainly comp.osed of 8 kinds of fatty acids and the content of saturated fatty acids is close to 20%（w/w）. This method is simple and fast, the analytic time is short and the result is accurate, which can provide reference for the similar products of fatty acid content.

关 键 词：红花籽 脂肪酸 气质联用 新疆产区 

分 类 号：O657.63[理学—分析化学]