脑立体定位微透析检测全脑缺血-再灌注模型羟自由基水平  

Quantitative evaluation of the hydroxyl radical level after global brain ischemia by intracranialmicrodialysis under stereotaxis

作　　者：王岩[1] 赵敏[1] 

机构地区：[1]中国医科大学附属盛京医院急诊科,沈阳110004

出　　处：《中华急诊医学杂志》2016年第7期887-891,共5页Chinese Journal of Emergency Medicine

基　　金：国家临床重点专科建设项目（2012649）

摘　　要：目的探讨两种供氧方式对于全脑缺血-再灌注模型脑内羟自由基水平影响。方法雄性长爪沙鼠16只，脑立体定位于右侧海马CA1区植入微透析探针，阻断再开放双侧颈总动脉（BCAO）建立全脑缺血-再灌注模型，气管插管机械通气，随机（随机数字法）分为常压常氧治疗组（NO）和常压纯氧治疗组（HO），每组各8只。通过微透析探针连续灌注含水杨酸钠的人工脑脊液并采样，高效液相色谱一电化学法检测微透析液中二羟基苯甲酸（DHBAs：2，3-DHBA和2。5-DHBA）含量。结果全脑缺血阶段，DHBAs浓度保持稳定；再灌注早期，NO及HO组微透析液中DHBAs水平均较基础水平显著升高（P〈0．05），且HO组较NO组升高更为显著（P〈0．05），维持更长时间。结论全脑缺血再灌早期阶段注脑内羟自由基的生成水平与氧暴露环境呈正相关。Objective To compare the strength of oxidative stress from different concentrations of oxygen administered by the accumulation of hydroxyl radicals during early reperfusion after global brain ischemia. Methods Sixteen adult male Mongolian gerbils with microdialysis probes implanted in the hippocampal CA1 were divided randomly （random number） into two groups （n = 8 in each）. All gerbils of both groups were subjected to 10 min bilateral carotid artery occlusion （BCAO）. Then the following intervention： （a） immediate 30% 02 （near normoxia, NO group） and （b） immediate 100% 02 （hyperoxia, HO group）. The accumulation of hydroxyl radicals （ · OH） in hippoeampus during reperfusion was estimated by measuring 2, 3-dihydroxybenzoic acid （DHBA） and 2, 5-DHBA in microdialysis perfusate. Resdts Immediately after the onset of reperfusion, two groups showed markedly elevated DHBA, which returned to baseline gradually. Compared with the NO group, the HO group showed significantly higher peak DHBA and slower recovery. Conclusions Hydroxyl radical accumulation was more sensitive to inhalation of high concentration 02 during early reperfusion of global cerebral ischemia.

关 键 词：全脑缺血-再灌注 氧治疗 脑立体定位微透析 羟自由基 长爪沙鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]