树莓干果粗黄酮纯化和活性研究  

A study on the purification and ctivity of total flavonoids from raspberry dry fruits

作　　者：武彦辉[1] 杨静[1,2] 原倩倩[1] 李娟玲 梅雪峰[1] 李小龙[1] 眭华梅[1] 

机构地区：[1]中北大学化工与环境学院,山西太原030051 [2]北京农学院农业部都市农业(北方)重点实验室,北京102206

出　　处：《云南大学学报（自然科学版）》2016年第4期676-682,共7页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学青年基金(31200462);山西省自然科学青年基金(2012021029-1);北京农学院农业部都市农业(北方)重点实验室开放课题(KFK2015001);大学生创新创业课题(2014-2016)

摘　　要：以树莓干果为原料,通过比较HP-20、D101、X-5、LX-68、AB-8、XDA-6、XDA-8、D201大孔树脂对树莓粗黄酮静态吸附率和解吸率的影响,筛选出适宜分离纯化树莓黄酮的大孔树脂为XDA-6树脂.结合静态与动态吸附解吸实验,得出用XDA-6大孔树脂分离纯化树莓黄酮的最佳工艺.将树莓粗黄酮提取原液作为上样液,以6 BV/h(1 BV为1个柱体积)的流速上样吸附,之后采用60%乙醇作为洗脱剂,以4 BV/h的流速进行洗脱,洗脱剂用量为5 BV.在此纯化条件下所得树莓黄酮质量分数为35.8%,较纯化前提高了1.21倍;干粉质量浓度在0.5 mg/m L时,对DPPH的抗氧化活性从纯化前的62.51%提高到70.36%,对大肠杆菌、金黄色葡萄球菌、棉花枯萎菌、小麦赤霉菌均有一定的抑制作用,纯化后的抑菌效果优于纯化前.By comparing static-adsorption and desorption rate of HP-20, D101, X-5, LX-68, AB-8, XDA- 6,XDA-8 and D201 macroporous resins,XDA-6 was considered to be the optimum resin to purify the crude fla- vonoids of raspberry dry fruits.By combining the static and dynamic experiments of resin adsorption-desorption, the optimum purification process was as follow：Taking crude flavonoids solution as sample,loading velocity at 6 BV/h （ 1 column volume as 1 BV） ,60 % alcohol as elution agent with 4 BV/h elution velocity, and 5 BV eluent volume.The purified flavonoids content by the process was 35.8 % ,which was 1.21 times more than that of the unpurified flavonoids solution.When dry mass concentration was 0.5 mg/mL, the antioxidant activity of total flavonoids to DPPH increased from 62.51% to 70.36 % after purification.The antibacterial effect of purified total flavonoids on Escherichia coli, Staphylococcus aureus, Cotton fusarium Wilt vasinfectum and Fusarium graminearum was better than unpurified flavonoids.

关 键 词：树莓干果 黄酮 纯化 抗氧化 抑菌 

分 类 号：S663.2[农业科学—果树学] Q944.5[农业科学—园艺学]