氟中毒病区人群血清中多种氧化应激指标的综合分析  

Comprehensive analysis of oxidative stress indexs in hunman serum with endemic fluorosis of coal burning

作　　者：陈修文[1] 万昌武[1] 官志忠[1] 吴昌学[1] 朱按英 李福成 唐福[2] 

机构地区：[1]贵阳医学院,贵州贵阳550004 [2]贵州省六盘水市疾病预防控制中心,贵州六盘水553000

出　　处：《工业卫生与职业病》2016年第4期249-252,共4页Industrial Health and Occupational Diseases

基　　金：国家科技支撑计划课题(2013BAI05B00);贵州省科技厅社发课题(2014黔科合SY字[2014]3024号)

摘　　要：目的观察氟中毒病区人群抗氧化能力及过氧化产物水平,为筛选氟中毒早期诊断指标提供依据。方法选取贵州省六盘水市陡箐乡氟病区165人为病区组,同市非病区花嘎乡90人为对照组,经体检及氟斑牙判定后采集空腹血液、尿液,分别检测尿氟、血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSH-Px)、总抗氧化能力(T-AOC)活力,同时检测血清中丙二醛(MDA)、8-异前列腺素F2α(8-isoPGF2α)、晚期氧化蛋白产物(AOPPs)和8-羟基脱氧鸟苷(8-OHdG)含量,分析氧化应激指标与氟斑牙及尿氟水平的相关性。结果病区组尿氟高于对照组(P<0.05),SOD、GSH-Px活力低于对照组(P<0.05),且与氟斑牙程度均呈负相关(P<0.05)。病区组血清中8-iso-PGF2α高于对照组(P<0.05),与氟斑牙程度呈正相关(P<0.05);病区组成人血清中AOPPs、8-OHdG高于对照组(P<0.05),但病区与非病区组儿童间比较,差异无统计学意义(P>0.05)。结论氟中毒病区人群普遍处于氧化应激状态,并存在脂质生物膜、蛋白质及DNA的多种损伤。Objective To observe the changes of the antioxidant ability and the levels of peroxidation products in hunman with endemic fluorosis of coal burning,and to provide the provement for early diagnosis of endemic fluorosis.Methods 165 residents were selected from Douqing town of Guiyang province,the endemic fluorosis of coal burning area,as the experimental group.90 residents were selected from Huaga town of Guiyang province,the non-endemic fluorosis of coal burning area as the control group.Urine and blood was collected on the empty stomach in the morning after medical examination and diagnosis of dental fluorosis.Flouride contents of urine,the activities of SOD,GSH-Px,T-AOC and contecnts of MDA,8-OhDG,8-iso-PGF2α,AOPPs in serum were measured respectively.Meanwhile the correlation between the activities and the degree of dental fluorosis and levels of the urinary fluoride should be analyzed.Results The flouride contents of urine in experimental group were significant higher than the control group（P〈0.05）.The activities of SOD,GSH-Px in experimental group were significant lower than the control group（P〈0.05）and showed negative correlations with the degree of dental fluorosis（P〈0.05）.The contents of 8-iso-PGF2αin experimental group were significant higher than the control group（P〈0.05）and showed positive correlations with the degree of dental fluorosis（P〈0.05）.The contents of 8-OHdG and AOPPs in adult experimental group were significant higher than the adult control group（P〈0.05）.There is no significant difference between the levels of T-AOC and MDA in the groups（P〉 0.05）.Conclusions The people with the the endemic fluorosis of coal burning were in the state of oxidative stress generally.In the meantime,the membrane,protein and DNA have been damaged.

关 键 词：氟中毒 氧化应激 抗氧化能力 过氧化产物 

分 类 号：R599[医药卫生—内科学]