油莎草实时荧光定量PCR内参基因的选择  

Reference Genes for Real-time Fluorescence Quantitative PCR in Cyperusesculentus. L

作　　者：敬思群[1] 于红[1] 郭改[1] 涂亦娴[1] 普燕[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《新疆大学学报（自然科学版）》2016年第3期265-269,379,共5页Journal of Xinjiang University(Natural Science Edition)

基　　金：国家自然科学基金-新疆联合基金项目(U1303103)资助

摘　　要：目的筛选不同生长时期的油莎草地上茎的内参基因,为分析其基因的表达提供支持.方法以生长40 d的油莎草幼苗10:00,12:00,14:00,16:00,18:00,20:00六个时间点的地上茎为材料;以及生长天数为20,30,40,50,55,65,75,85,95,105,115,125,135,145 d的油莎草地上茎为材料,采用实时荧光定量PCR(Quantitative real-time PCR,RT-q PCR)技术,分析其18S r RNA,GAPDH-两个候选基因.结论筛选出18S r RNA作为分析油莎草地上茎不同时期基因表达量的内参基因.[Objective] The objective of this experiment was to select reference gene of different growth periods of stems of Cyperusesculentus. L to support analysis of the gene expression. [Method] In this study, quantitative real-time PCR（RT-q PCR） was used to analyze the m RNA expression of two candidate reference genes, 18 S r RNA and GAPDH, in six time point（10：00,12：00,14：00,16：00,18：00,20：00） stems of Cyperusesculentus. L forty days and in stems of Cyperusesculentus.L seedlings grown different days（20, 30,40, 50, 55, 65, 75, 85, 95, 105, 115, 125, 135, 145 d）. [Result] 18 SrRNA was selected to reference gene of analyzing the gene expression of different growth periods.

关 键 词：油莎草 荧光定量PCR 内参基因 

分 类 号：Q786[生物学—分子生物学]