甲基硒代半胱氨酸上调硒结合蛋白1的表达对胃癌SGC7901细胞增殖的影响  

Effect of methylselenocysteine in up-regulation selenium- binding protein 1 for gastric carcinoma cells SGC7901 proliferation in vitro

作　　者：向琴 邹金艳[2] 易三凤[2] 商建[2] 林军[2] 

机构地区：[1]武汉市第五医院消化内科,湖北武汉430050 [2]武汉大学中南医院消化内科,湖北武汉430071

出　　处：《现代肿瘤医学》2016年第15期2343-2346,共4页Journal of Modern Oncology

基　　金：湖北省医学临床研究中心项目(编号:JX4D01);武汉市科技计划项目(编号:2014060101010054)

摘　　要：目的:研究甲基硒代半胱氨酸(MSC)对胃癌SGC7901细胞增殖的抑制作用,并寻找这种抑制作用与硒结合蛋白1(SBP1)之间的关系。方法:用不同浓度MSC处理胃癌SGC7901细胞,MTT法检测胃癌SGC7901细胞生长抑制率,实时荧光定量PCR(Real-time PCR)和免疫印迹(Western blot)技术分别检测SBP1 mRNA和蛋白水平的表达。结果:与对照组相比,MTT检测结果显示胃癌SGC7901细胞经浓度为25、50、75、100μmol/L的MSC处理后,体外增殖均受到抑制。Real-time PCR结果显示,25μmol/L浓度组MSC对SBP1mRNA表达影响无明显统计学意义;但50、75、100μmol/L浓度组SBP1 mRNA的表达均明显上调,在MSC有效浓度范围之内,随着MSC浓度的梯度增加,SBP1 mRNA的表达也相应上调。Western blot结果显示MSC分别作用24h、48h及72h后,各浓度组之间SBP1蛋白的相对表达量比较差异均有统计学意义,且随着MSC浓度的梯度增加,SBP1蛋白的相对表达量呈上升趋势。结论:MSC是一种低毒的含硒抗癌活性物质,它在干预胃癌中的作用并非基于对肿瘤细胞的直接毒性效应。MSC可能通过上调胃癌SGC7901细胞SBP1的表达而发挥抗癌作用。Objective： The study was designed to evaluate the effection of methylselenocysteine（ MSC） on antitumor and to delineate the underlying mechanism associated with observed in vitro between MSC and selenium- binding protein 1（ SBP1） in gastric carcinoma cells SGC7901. Methods： Cells were treated with different concentrations and schedules of MSC. The growth and proliferation rate were assessed with tetrazolium blue micro- ELISA assay（ MTT）.The expression of SBP1 mRNA and protein were measured with quantitative Real- time PCR and Western blot analyses,respectively. Results： Antiproliferative activities were expressed as drug concentrations that induced inhibition of cell growth compared with cell growth of untreated controls. Quantitative Real- time PCR and Western blot analysis indicated that MSC induced a statistically significant increase in SBP1 mRNA and protein expression as compared to the controls in most experiment groups except the 25μmol / L group in Real- time PCR analysis. Conclusion： Inhibiting cell proliferation is not the main mechanism of anticancer action of MSC in gastric carcinoma cells SGC7901. It was demonstrated that following the increasing of MSC concentration,SBP1 mRNA and protein expression were increased,which reversed the downregulation of SBP1 in gastric carcinoma cells SGC7901. These results provide evidence for a link between SBP1 and the mechanism of MSC on anticancer effection.

关 键 词：甲基硒代半胱氨酸 硒结合蛋白1 胃癌SGC7901细胞 

分 类 号：R735.2[医药卫生—肿瘤]