小鼠早期放射性肺损伤组织中单核细胞趋化蛋白1的表达及其意义  

Expression and significance of monocyte chemotactic protein 1 in early radiation-induced lung injury tissue of mice

作　　者：王贵富[1,2] 王宝中[2] 盛延兴[2] 张宪真[3] 

机构地区：[1]泰山医学院,山东泰安271000 [2]山东省聊城市人民医院放疗科,252000 [3]山东省聊城市人民医院肿瘤科,252000

出　　处：《肿瘤研究与临床》2016年第6期369-372,共4页Cancer Research and Clinic

基　　金：聊城市科技发展计划（2012NSl4）

摘　　要：目的研究小鼠早期放射性肺损伤过程中单核细胞趋化蛋白1（MCP-1）的表达及其意义。方法将40只C57BL／6小鼠按数字表法随机分为假照射组（10只）、照射组（30只），对照射组小鼠进行一次性大剂量（15Gy）胸部照射，分别于第1、10、30天处死照射组小鼠各10只，并收集标本。运用实时荧光定量聚合酶链反应（PCR）技术检测MCP-1 mRNA表达水平，酶联免疫吸附测定（ELISA）法检测支气管肺泡灌洗液中MCP-1、白细胞介素6（IL．6）含量。苏木精-伊红（HE）染色后光学显微镜下观察肺组织病理形态变化。结果光学显微镜下发现照射组照射后第10天肺组织出现炎性表现，第30天加重。免疫组织化学检测显示，照射后肺组织MCP-1蛋白表达增强。对照组、照射后第1、10、30天组的MCP-1 mRNA相对表达水平分别为7．63±0.77、6．81±0．75、5．75±0．73、4．61±0．75，照射后第1天组与对照组间、照射后第10天组与照射后第1天组间、照射后第30天组与照射后第10天组间MCP-1mRNA相对表达量差异均有统计学意义（均P〈0．05）。ELISA结果显示，照射组MCP-1、IL-6含量均较对照组明显升高（P〈0．05）。结论小鼠接受胸部照射后MCP-1表达异常，MCP-1可能参与了早期放射性肺损伤过程。Objective To study the expression and significance of monocyte chemotactic protein 1 （MCP-1） in the process of early radiation-induced lung injury in mice. Methods 40 mice were randomly divided into control group （10 cases） and post-irradiation group （30 cases）. A large dose （15 Gy） irradiation on chest was performed in post-irradiation group. Every 10 mice in this group were killed on the 1st day （RTld）, the 10th day （RT10d）, the 30th day （RT30d）, respectively, and the specimens were collected. The expression of MCP-1 mRNA was detected by quantitative real-time PCR （RT-PCR）, and MCP-1 and IL-6 proteins in BALF were detected by ELISA. The pathological changes of lung tissue were observed with HE staining. Results Under the microscope, lung tissue began to show inflammation on the 10th day and was more seriousness on the 30th day in post-irradiation group. Immunohistoehemistry staining showed that the expression of MCP-1 in lung tissue irradiated was increased. RT-PCR results showed that the relative expressions of MCP-1 mRNA in control group, RTld group, RT10d group and RT30d group were 7.63±0.77, 6.81±0.75, 5.75±0.73 and 4.61±0.75, respectively. The differences between RTld group and control group, RT10d and RTld group, and RT30d and RT10d were significantly different （all P 〈 0.05）. ELISA results showed that the levels of MCP-1 and IL-6 proteins in post-irradiation group were significantly higher than those in the control group （P 〈 0.05）. Conclusion The expression of MCP-1 in mice after irradiation is abnormal, and MCP-1 may participate in early radiation-induced lung injury.

关 键 词：辐射 肺损伤 单核细胞趋化蛋白1 白细胞介素6 

分 类 号：R730.55[医药卫生—肿瘤]